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Fluo-4, Pentapotassium Salt
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. Cells may be physically loaded with the cell-impermeant salt forms of these indicators using patch pipette or microinjection. These indicators are useful for fluorescence and confocal microscopy, flow cytometry, and microplate screening applications.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Fluo-4, Pentapotassium Salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 107.866 µL | 539.328 µL | 1.079 mL | 5.393 mL | 10.787 mL |
| 5 mM | 21.573 µL | 107.866 µL | 215.731 µL | 1.079 mL | 2.157 mL |
| 10 mM | 10.787 µL | 53.933 µL | 107.866 µL | 539.328 µL | 1.079 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
| Fluo-3, pentapotassium salt | 506 | 515 | 86,0001 | 0.151 |
| Fluo-3FF, pentapotassium salt | 506 | 515 | 86,0001 | 0.151 |
| Fluo-5F, pentapotassium Salt *Cell impermeant* | 494 | 516 | - | - |
| Fluo-5N, pentapotassium Salt *Cell impermeant* | 494 | 516 | - | - |
Citations
View all 1 citations: Citation Explorer
Succinate, increased in metabolic syndrome, activates GPR91 receptor signaling in urothelial cells
Authors: Mossa, Abubakr H and Flores, Monica Velasquez and Cammisotto, Philippe G and Campeau, Lysanne
Journal: Cellular Signalling (2017)
Authors: Mossa, Abubakr H and Flores, Monica Velasquez and Cammisotto, Philippe G and Campeau, Lysanne
Journal: Cellular Signalling (2017)
Page updated on December 17, 2024
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