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FITC–dextran conjugate (average MW = ~20K)
FITC-labeled dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. It is also used in microcirculation and cell permeability research utilizing microfluorimetry, and possible in perfusion studies in animals. FITC-dextran has also been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran. Small FITC-dextrans are often used to measure processes such as endocytosis and cell junction permeability. Fluorescein-dextran, 20kDa, may be used to study cell processes (such as endocytosis and osmoporation), endothelial or epithelial monolayer porosity, apical-basolateral movements within cell layers and cell junction permeability and drug release from structures such as hydrogens and collagen minirods.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) |
| FITC–dextran conjugate (average MW = ~70K) | 491 | 516 | 73000 | 0.92 | 0.35 |
| FITC–dextran conjugate (average MW = ~150K) | 491 | 516 | 73000 | 0.92 | 0.35 |
| TRITC–dextran conjugate (average MW = ~20K) | 544 | 570 | 100000 | - | 0.34 |
Citations
View all 44 citations: Citation Explorer
Vitexin Suppresses High-Glucose-upregulated Adhesion Molecule Expression in Endothelial Cells through Inhibiting NF-$\kappa$B Signaling Pathway
Authors: Chen, Pie-Che and Chang, Yun-Ching and Tsai, Kun-Ling and Shen, Cheng Huang and Lee, Shin-Da
Journal: ACS Omega (2024)
Authors: Chen, Pie-Che and Chang, Yun-Ching and Tsai, Kun-Ling and Shen, Cheng Huang and Lee, Shin-Da
Journal: ACS Omega (2024)
Lymphatic Reconnection and Restoration of Lymphatic Flow by Nonvascularized Lymph Node Transplantation: Real-Time Fluorescence Imaging Using Indocyanine Green and Fluorescein Isothiocyanate-Dextran
Authors: Maeda, T., Yamamoto, Y., Iwasaki, D., Hayashi, T., Funayama, E., Oyama, A., Murao, N., Furukawa, H.
Journal: Lymphat Res Biol (2018): 165-173
Authors: Maeda, T., Yamamoto, Y., Iwasaki, D., Hayashi, T., Funayama, E., Oyama, A., Murao, N., Furukawa, H.
Journal: Lymphat Res Biol (2018): 165-173
Optimizing Fluorescein Isothiocyanate Dextran Measurement As a Biomarker in a 24-h Feed Restriction Model to Induce Gut Permeability in Broiler Chickens
Authors: Baxter, M. F. A., Merino-Guzman, R., Latorre, J. D., Mahaffey, B. D., Yang, Y., Teague, K. D., Graham, L. E., Wolfenden, A. D., Hern and ez-Velasco, X., Bielke, L. R., Hargis, B. M., Tellez, G.
Journal: Front Vet Sci (2017): 56
Authors: Baxter, M. F. A., Merino-Guzman, R., Latorre, J. D., Mahaffey, B. D., Yang, Y., Teague, K. D., Graham, L. E., Wolfenden, A. D., Hern and ez-Velasco, X., Bielke, L. R., Hargis, B. M., Tellez, G.
Journal: Front Vet Sci (2017): 56
Fluorescein Isothiocyanate (FITC)-Dextran Extravasation as a Measure of Blood-Brain Barrier Permeability
Authors: Natarajan, R., Northrop, N., Yamamoto, B.
Journal: Curr Protoc Neurosci (2017): 9 58 1-9 58 15
Authors: Natarajan, R., Northrop, N., Yamamoto, B.
Journal: Curr Protoc Neurosci (2017): 9 58 1-9 58 15
Adenosine receptor agonist NECA increases cerebral extravasation of fluorescein and low molecular weight dextran independent of blood-brain barrier modulation
Authors: Cheng, C. C., Yang, Y. L., Liao, K. H., Lai, T. W.
Journal: Sci Rep (2016): 23882
Authors: Cheng, C. C., Yang, Y. L., Liao, K. H., Lai, T. W.
Journal: Sci Rep (2016): 23882
Page updated on November 21, 2024
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