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Digoxigenin azide
Digoxigenin (DIG) is a hapten, a small molecule with high antigenicity, which is used in many biological detections similarly to other popular haptens such as 2,4-Dinitrophenol (DNP) and biotin. Typically, digoxigenin is conjugated to a macrobiomolecule (e.g., proteins, nucleic acids, etc) to be detected. Anti-digoxigenin antibodies are labeled with fluorescent dyes or enzymes, directly or secondarily, for visualization and detection. Digoxigenin azide readily reacts with alkyne group under mild conditions, attaching the digoxigenin moiety to alkyne-containing molecules. Digoxigenin-labled biomolecules are used for biological detections in combination with anti-digoxigenin antibodies of high affinity and specificity.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Digoxigenin azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 193.558 µL | 967.792 µL | 1.936 mL | 9.678 mL | 19.356 mL |
| 5 mM | 38.712 µL | 193.558 µL | 387.117 µL | 1.936 mL | 3.871 mL |
| 10 mM | 19.356 µL | 96.779 µL | 193.558 µL | 967.792 µL | 1.936 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| AMCA Azide | 346 | 434 | 19000 | 0.153 |
| XFD488 azide *Same Structure to Alexa Fluor™ 488 azide* | 499 | 520 | 71000 | 0.11 |
| ICG azide | 789 | 813 | 230000 | 0.076 |
| Cy3B azide | 560 | 571 | 1200001 | 0.069 |
| XFD647 Azide | 650 | 671 | 270000 | 0.03 |
| XFD350 azide | 343 | 441 | 19000 | 0.19 |
| XFD405 azide | 401 | 421 | 35,000 | 0.70 |
| XFD430 azide | 432 | 540 | 15,000 | 0.28 |
| XFD514 azide | 518 | 543 | 80000 | 0.18 |
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Citations
View all 21 citations: Citation Explorer
LncRNA AL592284. 1 facilitates proliferation and metastasis of cervical cancer cells via miR-30a-5p/Vimentin/EMT axis
Authors: Zhang, Jing and Liu, Hong-li and Liu, Jing-bo and Zhang, Yuan and Liu, Jing and Li, Yan-hua
Journal: Biochemical and Biophysical Research Communications (2021): 95--102
Authors: Zhang, Jing and Liu, Hong-li and Liu, Jing-bo and Zhang, Yuan and Liu, Jing and Li, Yan-hua
Journal: Biochemical and Biophysical Research Communications (2021): 95--102
A smartphone readout system for gold nanoparticle-based lateral flow assays: application to monitoring of digoxigenin
Authors: Ruppert, C., Phogat, N., Laufer, S., Kohl, M., Deigner, H. P.
Journal: Mikrochim Acta (2019): 119
Authors: Ruppert, C., Phogat, N., Laufer, S., Kohl, M., Deigner, H. P.
Journal: Mikrochim Acta (2019): 119
Application of Antibody-Powered Triplex-DNA Nanomachine to Electrochemiluminescence Biosensor for the Detection of Anti-Digoxigenin with Improved Sensitivity Versus Cycling Strand Displacement Reaction
Authors: Yang, S. S., Jiang, M. H., Chai, Y. Q., Yuan, R., Zhuo, Y.
Journal: ACS Appl Mater Interfaces (2018): 38648-38655
Authors: Yang, S. S., Jiang, M. H., Chai, Y. Q., Yuan, R., Zhuo, Y.
Journal: ACS Appl Mater Interfaces (2018): 38648-38655
Synthesis of C3-Neoglycosides of digoxigenin and their anticancer activities
Authors: Li, X. S., Ren, Y. C., Bao, Y. Z., Liu, J., Zhang, X. K., Zhang, Y. W., Sun, X. L., Yao, X. S., Tang, J. S.
Journal: Eur J Med Chem (2018): 252-262
Authors: Li, X. S., Ren, Y. C., Bao, Y. Z., Liu, J., Zhang, X. K., Zhang, Y. W., Sun, X. L., Yao, X. S., Tang, J. S.
Journal: Eur J Med Chem (2018): 252-262
The cardenolides strophanthidin, digoxigenin and dihydroouabain act as activators of the human RORgamma/RORgammaT receptors
Authors: Karas, K., Salkowska, A., Walczak-Drzewiecka, A., Ryba, K., Dastych, J., Bachorz, R. A., Ratajewski, M.
Journal: Toxicol Lett (2018): 314-324
Authors: Karas, K., Salkowska, A., Walczak-Drzewiecka, A., Ryba, K., Dastych, J., Bachorz, R. A., Ratajewski, M.
Journal: Toxicol Lett (2018): 314-324
Page updated on November 20, 2024
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