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DASPEI [2-(4-(dimethylamino)styryl)-N-ethylpyridinium iodide] *CAS#: 3785-01-1*
DASPEI is a styryl dye that stains mitochondria of live cells. This dye has a large fluorescence Stokes shift and is taken up relatively slowly as a function of membrane. The vital mitochondrial dye DASPEI has been used for the rapid visualization of several distinct classes of epidermal cells in vivo and in vitro: epidermal electroreceptors, mechanoreceptors, and chloride cells in teleosts, and mechanoreceptors in amphibians. DASPEI is also used to locate a different type of sensory cells. In vivo, bath-applied DASPEI stains entire larvae in a relatively non-specific fashion.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DASPEI [2-(4-(dimethylamino)styryl)-N-ethylpyridinium iodide] *CAS#: 3785-01-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 262.971 µL | 1.315 mL | 2.63 mL | 13.149 mL | 26.297 mL |
| 5 mM | 52.594 µL | 262.971 µL | 525.942 µL | 2.63 mL | 5.259 mL |
| 10 mM | 26.297 µL | 131.486 µL | 262.971 µL | 1.315 mL | 2.63 mL |
Molarity calculator
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Citations
View all 2 citations: Citation Explorer
Chemical screen in zebrafish lateral line identified compounds that ameliorate neomycin-induced ototoxicity by inhibiting ferroptosis pathway
Authors: Fan, Yipu and Zhang, Yihan and Qin, Dajiang and Shu, Xiaodong
Journal: Cell \& Bioscience (2024): 1--16
Authors: Fan, Yipu and Zhang, Yihan and Qin, Dajiang and Shu, Xiaodong
Journal: Cell \& Bioscience (2024): 1--16
Environmental Hazard Potential of Nano-Photocatalysts Determined by Nano-Bio Interactions and Exposure Conditions
Authors: He, Yuan and Peng, Guotao and Jiang, Yue and Zhao, Mei and Wang, Xiaoxiao and Chen, Mengmeng and Lin, Sijie
Journal: Small (2020): 1907690
Authors: He, Yuan and Peng, Guotao and Jiang, Yue and Zhao, Mei and Wang, Xiaoxiao and Chen, Mengmeng and Lin, Sijie
Journal: Small (2020): 1907690
References
View all 17 references: Citation Explorer
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Authors: Cai H, Rose K, Liang LH, Dunham S, Stover C.
Journal: Anal Biochem (2009): 321
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Authors: Jonz MG, Nurse CA.
Journal: J Comp Neurol (2006): 817
Authors: Jonz MG, Nurse CA.
Journal: J Comp Neurol (2006): 817
The use of zebrafish for assessing ototoxic and otoprotective agents
Authors: Ton C, Parng C.
Journal: Hear Res (2005): 79
Authors: Ton C, Parng C.
Journal: Hear Res (2005): 79
Mitochondria-rich cell activity in the yolk-sac membrane of tilapia (Oreochromis mossambicus) larvae acclimatized to different ambient chloride levels
Authors: Lin LY, Hwang PP.
Journal: J Exp Biol (2004): 1335
Authors: Lin LY, Hwang PP.
Journal: J Exp Biol (2004): 1335
Extramembrane central pore of multidrug exporter AcrB in Escherichia coli plays an important role in drug transport
Authors: Murakami S, Tamura N, Saito A, Hirata T, Yamaguchi A.
Journal: J Biol Chem (2004): 3743
Authors: Murakami S, Tamura N, Saito A, Hirata T, Yamaguchi A.
Journal: J Biol Chem (2004): 3743
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