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D-Luciferin phosphate *CAS 145613-12-3*

D-Luciferin phosphate is a highly sensitive bioluminescent substrate for detecting alkaline phosphatases and protein phosphatases by luminometric detection. It is used in combination with luciferase/ATP system. D-Luciferin phosphate can be converted to D-Luciferin by phosphatases (such as alkaline phosphatases, acid phosphatases and protein phosphatases) that gives luminescence in the presence of luciferase and its cofactors. Our Amplite luciferase assay kit can be used for detecting D-luciferin release from D-Luciferin phosphate by phosphatases.

Example protocol

AT A GLANCE

Important notes
It is important to store at <-15 °C and should be stored in cool, dark place.

It can be used within 12 months from the date of receipt.

PREPARATION OF WORKING SOLUTION

Working solution should be prepared in water or DMSO.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of D-Luciferin phosphate *CAS 145613-12-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM234.604 µL1.173 mL2.346 mL11.73 mL23.46 mL
5 mM46.921 µL234.604 µL469.208 µL2.346 mL4.692 mL
10 mM23.46 µL117.302 µL234.604 µL1.173 mL2.346 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum

Product family

NameExcitation (nm)Emission (nm)
D-Luciferin galactoside *CAS 131474-38-9*385529
D-Luciferin acetate385529

Citations

View all 6 citations: Citation Explorer
Extracellular vesicle surface display of $\alpha$PD-L1 and $\alpha$CD3 antibodies via engineered late domain-based scaffold to activate T-cell anti-tumor immunity
Authors: Chen, Rui and Kang, Ziqin and Li, Wenhao and Xu, Tianshu and Wang, Yongqiang and Jiang, Qiming and Wang, Yuepeng and Huang, Zixian and Xu, Xiaoding and Huang, Zhiquan
Journal: Journal of Extracellular Vesicles (2024): e12490
Bmal1 integrates circadian function and temperature sensing in the suprachiasmatic nucleus
Authors: Hoekstra, Marieke MB and Ness, Natalie and Badia-Soteras, Aina and Brancaccio, Marco
Journal: Proceedings of the National Academy of Sciences (2024): e2316646121
GRAS family member LATERAL SUPPRESSOR regulates the initiation and morphogenesis of watermelon lateral organs
Authors: Jiang, Yanxin and Zhang, Anran and He, Wenjing and Li, Qingqing and Zhao, Bosi and Zhao, Hongjiao and Ke, Xubo and Guo, Yalu and Sun, Piaoyun and Yang, Tongwen and others,
Journal: Plant Physiology (2023): kiad445
Design, synthesis, and biological evaluation studies of novel carboxylesterase 2 inhibitors for the treatment of irinotecan-induced delayed diarrhea
Authors: Yang, Zhongcheng and Cao, Zhijun and Wang, Wenxin and Chen, Ya and Huang, Wanqiu and Jiao, Shixuan and Chen, Siliang and Chen, Lianru and Liu, Yuxia and Mao, Jianming and others,
Journal: Bioorganic Chemistry (2023): 106625
Diltiazem inhibits breast cancer metastasis via mediating growth differentiation factor 15 and epithelial-mesenchymal transition
Authors: Chen, Yen-Chang and Wu, Chen-Teng and Chen, Jia-Hong and Tsai, Cheng-Fang and Wu, Chen-Yun and Chang, Pei-Chun and Yeh, Wei-Lan
Journal: Oncogenesis (2022): 1--9

References

View all 22 references: Citation Explorer
Antivascular effects of combretastatin A4 phosphate in breast cancer xenograft assessed using dynamic bioluminescence imaging and confirmed by MRI
Authors: Zhao D, Richer E, Antich PP, Mason RP.
Journal: Faseb J. (2008)
Mechanism of the stress-induced collapse of the Ran distribution
Authors: Yasuda Y, Miyamoto Y, Saiwaki T, Yoneda Y.
Journal: Exp Cell Res (2006): 512
Creatine phosphate administration preserves myocardial function in a model of off-pump coronary revascularization
Authors: Woo YJ, Gr and TJ, Zentko S, Cohen JE, Hsu V, Atluri P, Berry MF, Taylor MD, Moise MA, Fisher O, Kolakowski S.
Journal: J Cardiovasc Surg (Torino) (2005): 297
Human keratinocytes release ATP and utilize three mechanisms for nucleotide interconversion at the cell surface
Authors: Burrell HE, Wlodarski B, Foster BJ, Buckley KA, Sharpe GR, Quayle JM, Simpson AW, Gallagher JA.
Journal: J Biol Chem (2005): 29667
Mechanically driven ATP synthesis by F1-ATPase
Authors: Itoh H, Takahashi A, Adachi K, Noji H, Yasuda R, Yoshida M, Kinosita K.
Journal: Nature (2004): 465
Page updated on October 31, 2024

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Physical properties

Molecular weight

426.25

Solvent

Water

Spectral properties

Excitation (nm)

385

Emission (nm)

529

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC41105331

CAS

145613-12-3