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CytoWatch™ QZ100 Monocyte Blocking Reagent
Some dye-labeled fluorescent antibody conjugates used in cell surface staining of live cells often exhibit non-specific binding in monocytes and macrophages. CytoWatch™ QZ100 Monocyte Blocking Reagent is a non-antibody-based blocking solution that is optimized to blocking the non-specific background of cyanine-based dye conjugates. It can effectively eliminate non-specific staining of monocytes and macrophages by cyanine dye conjugates (such as PE/Cy5, PE/Cy7, APC/Cy7, PE/Dazzle™ 594, APC/Fire™ 750, PE/Alexa Fluor® 647, PE/Alexa Fluor® 750, APC/Alexa Fluor® 750, PE/iFluor® 647, PE/iFluor® 750 and APC/iFluor® 750). The reagent has no impact on the specific surface staining of live lymphocytes, monocytes, and granulocytes.
Example protocol
SAMPLE EXPERIMENTAL PROTOCOL
Cell Surface Staining Protocol for Flow Cytometry Analysis
- Add 5 µL of CytoWatch™ QZ100 Monocyte Blocking Reagent to 100 µL of PBMC or whole blood.
- Incubate for 5-10 minutes at room temperature or add primary antibodies immediately, and incubate at room temperature for 20 minutes.
- Wash twice with cell staining buffer.
- Resuspend cells in 0.5 mL of cell staining buffer.
- Perform flow cytometric analysis.
References
View all 50 references: Citation Explorer
Synergistic reduction in albuminuria in type 2 diabetic mice by esaxerenone (CS-3150), a novel nonsteroidal selective mineralocorticoid receptor blocker, combined with an angiotensin II receptor blocker.
Authors: Arai, Kiyoshi and Morikawa, Yuka and Ubukata, Naoko and Sugimoto, Kotaro
Journal: Hypertension research : official journal of the Japanese Society of Hypertension (2020): 1204-1213
Authors: Arai, Kiyoshi and Morikawa, Yuka and Ubukata, Naoko and Sugimoto, Kotaro
Journal: Hypertension research : official journal of the Japanese Society of Hypertension (2020): 1204-1213
Prior β-blocker treatment decreases leukocyte responsiveness to injury.
Authors: Grisanti, Laurel A and de Lucia, Claudio and Thomas, Toby P and Stark, Aron and Strony, John T and Myers, Valerie D and Beretta, Remus and Yu, Daohai and Sardu, Celestino and Marfella, Raffaele and Gao, Erhe and Houser, Steven R and Koch, Walter J and Hamad, Eman A and Tilley, Douglas G
Journal: JCI insight (2019)
Authors: Grisanti, Laurel A and de Lucia, Claudio and Thomas, Toby P and Stark, Aron and Strony, John T and Myers, Valerie D and Beretta, Remus and Yu, Daohai and Sardu, Celestino and Marfella, Raffaele and Gao, Erhe and Houser, Steven R and Koch, Walter J and Hamad, Eman A and Tilley, Douglas G
Journal: JCI insight (2019)
The Angiotensin Receptor Blocker Losartan Suppresses Growth of Pulmonary Metastases via AT1R-Independent Inhibition of CCR2 Signaling and Monocyte Recruitment.
Authors: Regan, Daniel P and Coy, Jonathan W and Chahal, Kirti Kandhwal and Chow, Lyndah and Kurihara, Jade N and Guth, Amanda M and Kufareva, Irina and Dow, Steven W
Journal: Journal of immunology (Baltimore, Md. : 1950) (2019): 3087-3102
Authors: Regan, Daniel P and Coy, Jonathan W and Chahal, Kirti Kandhwal and Chow, Lyndah and Kurihara, Jade N and Guth, Amanda M and Kufareva, Irina and Dow, Steven W
Journal: Journal of immunology (Baltimore, Md. : 1950) (2019): 3087-3102
Neprilysin Inhibitor-Angiotensin II Receptor Blocker Combination Therapy (Sacubitril/valsartan) Suppresses Atherosclerotic Plaque Formation and Inhibits Inflammation in Apolipoprotein E- Deficient Mice.
Authors: Zhang, Hui and Liu, Gangqiong and Zhou, Wenping and Zhang, Wenjing and Wang, Kai and Zhang, Jinying
Journal: Scientific reports (2019): 6509
Authors: Zhang, Hui and Liu, Gangqiong and Zhou, Wenping and Zhang, Wenjing and Wang, Kai and Zhang, Jinying
Journal: Scientific reports (2019): 6509
Angiotensin II type 1 receptor blocker telmisartan induces apoptosis and autophagy in adult T-cell leukemia cells.
Authors: Kozako, Tomohiro and Soeda, Shuhei and Yoshimitsu, Makoto and Arima, Naomichi and Kuroki, Ayako and Hirata, Shinya and Tanaka, Hiroaki and Imakyure, Osamu and Tone, Nanako and Honda, Shin-Ichiro and Soeda, Shinji
Journal: FEBS open bio (2016): 442-60
Authors: Kozako, Tomohiro and Soeda, Shuhei and Yoshimitsu, Makoto and Arima, Naomichi and Kuroki, Ayako and Hirata, Shinya and Tanaka, Hiroaki and Imakyure, Osamu and Tone, Nanako and Honda, Shin-Ichiro and Soeda, Shinji
Journal: FEBS open bio (2016): 442-60
Page updated on November 21, 2024
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