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CytoWatch™ Multiplexing Buffer for Flow Cytometry *20X*
Fluorescent antibody conjugates tend to aggregate when in close contact, distorting the analytical results of multicolor staining cells due to FRET and other energy transfer phenomena between different conjugates. Fluorescent dye interactions may cause staining artifacts, complicating the data interpretation. CytoWatch™ Multiplexing Buffer is a buffer optimized for the multicolor flow cytometric analysis of cells. CytoWatch™ Multiplexing Buffer is a concentrated solution that is directly added to the staining solutions of certain fluorescent antibody conjugates or other fluorescent reagents before staining cells. It might be used to enhance or improve multicolor flow cytometry experiments that utilize two or more different staining reagents (e.g., antibodies conjugated with BV dyes or Qdots).
Example protocol
SAMPLE EXPERIMENTAL PROTOCOL
Cell Surface Staining Protocol for Flow Cytometry Analysis:
- Add 5 µL of CytoWatch™ Multiplexing Buffer to 100 µL of PBMC. Mix well by pipetting up and down several times.
- Add appropriate amounts of conjugated antibodies.
- Mix well by pipetting up and down and stain at room temperature or 2~8°C for 20 minutes.
- Wash twice with a flow cytometric cell staining buffer of your choice.
- Resuspend cells in an appropriate volume of flow cytometric cell staining buffer.
- Perform flow cytometric analysis.
References
View all 46 references: Citation Explorer
Azo dyes decolorization under high alkalinity and salinity conditions by Halomonas sp. in batch and packed bed reactor.
Authors: Montañez-Barragán, B and Sanz-Martín, J L and Gutiérrez-Macías, P and Morato-Cerro, A and Rodríguez-Vázquez, R and Barragán-Huerta, B E
Journal: Extremophiles : life under extreme conditions (2020): 239-247
Authors: Montañez-Barragán, B and Sanz-Martín, J L and Gutiérrez-Macías, P and Morato-Cerro, A and Rodríguez-Vázquez, R and Barragán-Huerta, B E
Journal: Extremophiles : life under extreme conditions (2020): 239-247
Adsorption of Remazol Brilliant Violet-5R Textile Dye from Aqueous Solutions by Using Eggshell Waste Biosorbent.
Authors: Rápó, Eszter and Aradi, László Előd and Szabó, Ábel and Posta, Katalin and Szép, Robert and Tonk, Szende
Journal: Scientific reports (2020): 8385
Authors: Rápó, Eszter and Aradi, László Előd and Szabó, Ábel and Posta, Katalin and Szép, Robert and Tonk, Szende
Journal: Scientific reports (2020): 8385
Process optimization and filtration performance of an anaerobic dynamic membrane bioreactor treating textile wastewaters.
Authors: Yurtsever, Adem and Basaran, Erkan and Ucar, Deniz
Journal: Journal of environmental management (2020): 111114
Authors: Yurtsever, Adem and Basaran, Erkan and Ucar, Deniz
Journal: Journal of environmental management (2020): 111114
Simultaneous Polychromatic Immunofluorescent Staining of Tissue Sections and Consecutive Imaging of up to Seven Parameters by Standard Confocal Microscopy.
Authors: Schmidt, Alfonso J and Mayer, Johannes U and Wallace, Paul K and Ronchese, Franca and Price, Kylie M
Journal: Current protocols in cytometry (2019): e64
Authors: Schmidt, Alfonso J and Mayer, Johannes U and Wallace, Paul K and Ronchese, Franca and Price, Kylie M
Journal: Current protocols in cytometry (2019): e64
Remediation of complex remazol effluent using biochar derived from green seaweed biomass.
Authors: Gokulan, Ravindiran and Prabhu, Ganapathy Ganesh and Jegan, Josephraj
Journal: International journal of phytoremediation (2019): 1179-1189
Authors: Gokulan, Ravindiran and Prabhu, Ganapathy Ganesh and Jegan, Josephraj
Journal: International journal of phytoremediation (2019): 1179-1189
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