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CytoTrace™ Red CFDA
CytoTrace™ Red fluorescent probe retains the spectral properties of Cy3/TRITC, thus its signal can be easily monitored using fluorescence microscopy or flow cytometry. It freely passes through cell membranes and is converted to cell-impermeant products upon reactions with cellular components. The cell-impermeant reaction product is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CytoTrace™ Red probe is typically fluorescent and viable for at least 24 hours, making this probe an excellent long-term cell tracer. The staining pattern can be fixed with formaldehyde or glutaraldehyde for signal amplification and other applications. Its spectra are well separated from those of GFP or FITC-labeled antibodies, making it an excellent choice for multiplexing with GFP cell lines or FITC-labeled antibodies.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of CytoTrace™ Red CFDA to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 153.273 µL | 766.366 µL | 1.533 mL | 7.664 mL | 15.327 mL |
| 5 mM | 30.655 µL | 153.273 µL | 306.546 µL | 1.533 mL | 3.065 mL |
| 10 mM | 15.327 µL | 76.637 µL | 153.273 µL | 766.366 µL | 1.533 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Citations
View all 3 citations: Citation Explorer
Exploring the Functions of Mutant p53 through TP53 Knockout in HaCaT Keratinocytes
Authors: Romashin, Daniil and Rusanov, Alexander and Arzumanian, Viktoriia and Varshaver, Alexandra and Poverennaya, Ekaterina and Vakhrushev, Igor and Netrusov, Alexander and Luzgina, Nataliya
Journal: Current Issues in Molecular Biology (2024): 1451--1466
Authors: Romashin, Daniil and Rusanov, Alexander and Arzumanian, Viktoriia and Varshaver, Alexandra and Poverennaya, Ekaterina and Vakhrushev, Igor and Netrusov, Alexander and Luzgina, Nataliya
Journal: Current Issues in Molecular Biology (2024): 1451--1466
Biodegradable Mg alloy modified with bioactive exosomes for cardiovascular stent application
Authors: Hou, Ya-chen and Li, Jing-an and Cao, Chang and Su, Chang and Qin, Zhen and Zhang, Ge and Guo, Jia-cheng and Tang, Jun-nan and Zhang, Jin-ying and Guan, Shao-kang
Journal: Journal of Magnesium and Alloys (2023)
Authors: Hou, Ya-chen and Li, Jing-an and Cao, Chang and Su, Chang and Qin, Zhen and Zhang, Ge and Guo, Jia-cheng and Tang, Jun-nan and Zhang, Jin-ying and Guan, Shao-kang
Journal: Journal of Magnesium and Alloys (2023)
The increased ratio of Mg2+/Ca2+ from degrading magnesium alloys directs macrophage fate for functionalized growth of endothelial cells
Authors: Hou, Ya-chen and Witte, Frank and Li, Jingan and Guan, Shaokang
Journal: Smart Materials in Medicine (2022)
Authors: Hou, Ya-chen and Witte, Frank and Li, Jingan and Guan, Shaokang
Journal: Smart Materials in Medicine (2022)
References
View all 23 references: Citation Explorer
Fluorescence-Based Transport Assays Revisited in a Human Renal Proximal Tubule Cell Line
Authors: Caetano-Pinto P, Janssen MJ, Gijzen L, Verscheijden L, Wilmer MJ, Masereeuw R.
Journal: Mol Pharm (2016): 933
Authors: Caetano-Pinto P, Janssen MJ, Gijzen L, Verscheijden L, Wilmer MJ, Masereeuw R.
Journal: Mol Pharm (2016): 933
The variable chemotherapeutic response of Malabaricone-A in leukemic and solid tumor cell lines depends on the degree of redox imbalance
Authors: Manna A, De Sarkar S, De S, Bauri AK, Chattopadhyay S, Chatterjee M.
Journal: Phytomedicine (2015): 713
Authors: Manna A, De Sarkar S, De S, Bauri AK, Chattopadhyay S, Chatterjee M.
Journal: Phytomedicine (2015): 713
Cell membrane tracker based on restriction of intramolecular rotation
Authors: Zhang C, Jin S, Yang K, Xue X, Li Z, Jiang Y, Chen WQ, Dai L, Zou G, Liang XJ.
Journal: ACS Appl Mater Interfaces (2014): 8971
Authors: Zhang C, Jin S, Yang K, Xue X, Li Z, Jiang Y, Chen WQ, Dai L, Zou G, Liang XJ.
Journal: ACS Appl Mater Interfaces (2014): 8971
Evaluation of stability and sensitivity of cell fluorescent labels when used for cell migration
Authors: Beem E, Segal MS.
Journal: J Fluoresc (2013): 975
Authors: Beem E, Segal MS.
Journal: J Fluoresc (2013): 975
A multiple model probability hypothesis density tracker for time-lapse cell microscopy sequences
Authors: Rezatofighi SH, Gould S, Vo BN, Mele K, Hughes WE, Hartley R.
Journal: Inf Process Med Imaging (2013): 110
Authors: Rezatofighi SH, Gould S, Vo BN, Mele K, Hughes WE, Hartley R.
Journal: Inf Process Med Imaging (2013): 110
Page updated on December 17, 2024
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