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CytoTrace™ Orange CMTMR *CAS 323192-14-9*
CytoTrace™ Orange CMTMR is chemically same to CellTracker™ Orange CMTMR. CytoTrace™ Orange CMTMR freely passes through cell membranes into cells, where it is transformed into cell membrane-impermeant reaction products. CytoTrace™ Orange CMTMR dye is retained in living cells through several generations. The dye is transferred to daughter cells, but not adjacent cells in a population. CytoTrace™ Orange CMTMR dye is designed to display fluorescence for at least 72 hours, and the dye exhibits ideal tracking properties. CMTMR dye is stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, the excitation and emission spectra of CytoTrace™ Orange CMTMR dye are well separated from GFP (green fluorescent protein) and spectra allowing for multiplexing.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of CytoTrace™ Orange CMTMR *CAS 323192-14-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 180.492 µL | 902.462 µL | 1.805 mL | 9.025 mL | 18.049 mL |
| 5 mM | 36.098 µL | 180.492 µL | 360.985 µL | 1.805 mL | 3.61 mL |
| 10 mM | 18.049 µL | 90.246 µL | 180.492 µL | 902.462 µL | 1.805 mL |
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References
View all 23 references: Citation Explorer
Fluorescence-Based Transport Assays Revisited in a Human Renal Proximal Tubule Cell Line
Authors: Caetano-Pinto P, Janssen MJ, Gijzen L, Verscheijden L, Wilmer MJ, Masereeuw R.
Journal: Mol Pharm (2016): 933
Authors: Caetano-Pinto P, Janssen MJ, Gijzen L, Verscheijden L, Wilmer MJ, Masereeuw R.
Journal: Mol Pharm (2016): 933
The variable chemotherapeutic response of Malabaricone-A in leukemic and solid tumor cell lines depends on the degree of redox imbalance
Authors: Manna A, De Sarkar S, De S, Bauri AK, Chattopadhyay S, Chatterjee M.
Journal: Phytomedicine (2015): 713
Authors: Manna A, De Sarkar S, De S, Bauri AK, Chattopadhyay S, Chatterjee M.
Journal: Phytomedicine (2015): 713
Cell membrane tracker based on restriction of intramolecular rotation
Authors: Zhang C, Jin S, Yang K, Xue X, Li Z, Jiang Y, Chen WQ, Dai L, Zou G, Liang XJ.
Journal: ACS Appl Mater Interfaces (2014): 8971
Authors: Zhang C, Jin S, Yang K, Xue X, Li Z, Jiang Y, Chen WQ, Dai L, Zou G, Liang XJ.
Journal: ACS Appl Mater Interfaces (2014): 8971
Evaluation of stability and sensitivity of cell fluorescent labels when used for cell migration
Authors: Beem E, Segal MS.
Journal: J Fluoresc (2013): 975
Authors: Beem E, Segal MS.
Journal: J Fluoresc (2013): 975
A multiple model probability hypothesis density tracker for time-lapse cell microscopy sequences
Authors: Rezatofighi SH, Gould S, Vo BN, Mele K, Hughes WE, Hartley R.
Journal: Inf Process Med Imaging (2013): 110
Authors: Rezatofighi SH, Gould S, Vo BN, Mele K, Hughes WE, Hartley R.
Journal: Inf Process Med Imaging (2013): 110
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