Cell Navigator® Fluorimetric Lipid Droplet Assay Kit *Green Fluorescence*
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Correction Factor (260 nm) | 0.015 |
Correction Factor (280 nm) | 0.018 |
Extinction coefficient (cm -1 M -1) | 81000 |
Excitation (nm) | 504 |
Emission (nm) | 510 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
UNSPSC | 12352200 |
Cell Navigator® Fluorimetric Lipid Droplet Assay Kit *Red Fluorescence* |
Overview | ![]() ![]() |
Correction Factor (260 nm) 0.015 | Correction Factor (280 nm) 0.018 | Extinction coefficient (cm -1 M -1) 81000 | Excitation (nm) 504 | Emission (nm) 510 |
Platform
Fluorescence microscope
Excitation | FITC filter set |
Emission | FITC filter set |
Recommended plate | Black wall/clear bottom |
Fluorescence microplate reader
Excitation | 485 nm |
Emission | 520 nm |
Cutoff | 510 nm |
Recommended plate | Black wall/clear bottom |
Instrument specification(s) | Bottom read mode |
Components
Example protocol
AT A GLANCE
Protocol summary
- Prepare cells with test compounds
- Add Nile Green™ working solution
- Incubate at room temperature or 37°C for 10 to 30 min
- Read fluorescence intensity with fluorescence microscope using FITC filter
Important notes
Following is our recommended protocol for live cells. This protocol only provides a guideline, and should be modified according to your specific needs. Since Nile Green™ has minimal fluorescence in aqueous media, aspiration of the growth medium and removal of Nile Green™ staining solution after staining is optional. Stained cells can be fixed with 3 - 4% formaldehyde. In addition, prefixed cells (3 - 4% formaldehyde fixation) can be stained with Nile Green™ staining solution.
PREPARATION OF WORKING SOLUTION
Prepare Nile Green™ working solution by diluting 5 µL of 200X Nile Green™ (Component A) to 1 mL of Staining Buffer (Component B). Note: 50 µL of Nile Green™ (Component A) is enough for one 96-well plate. Protect from light. The optimal concentration of the Nile Green™ varies depending on specific applications. The staining conditions may be modified according to a particular cell type and the permeability of the cells or tissues to the probe.
For guidelines on cell sample preparation, please visit
https://www.aatbio.com/resources/guides/cell-sample-preparation.html
SAMPLE EXPERIMENTAL PROTOCOL
For adherent cells:
- Grow cells either in a 96-well black wall/clear bottom plate (100 µL/well/96-well) or on cover-slips inside a petri dish filled with the appropriate culture medium.
- Gently aspirate the culture medium and add equal volume (such as 100 µL/well/96-well plate) of the Nile Green™ staining solution.
- Incubate the cells in a 37°C, 5% CO2 incubator for 10 - 30 minutes.
- Remove Nile Green™ working solution (Optional).
- Read Fluorescence at 485/520 nm with a microplate reader or observe the cells using a fluorescence microscope with a FITC filter set.
For suspension cells:
- Centrifuge the cells at 1000 rpm for 5 minutes to get 1 - 5 × 105 cells per tube.
- Resuspend cells in 500 µL of Nile Green™ working solution.
- Incubate at room temperature or 37°C for 10 to 30 min, protected from light.
- Centrifuge to remove the Nile Green™ working solution, and resuspend cells in 500 µL of pre-warmed medium or buffer of your choice to get 1 - 5 × 105 cells per tube (Optional).
- Monitor the fluorescence increase using fluorescence microscope with a FITC filter set.
Spectrum
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Fbodi_fluor_488.png&w=2048&q=50)
Spectral properties
Correction Factor (260 nm) | 0.015 |
Correction Factor (280 nm) | 0.018 |
Extinction coefficient (cm -1 M -1) | 81000 |
Excitation (nm) | 504 |
Emission (nm) | 510 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
Cell Navigator® Fluorimetric Lipid Droplet Assay Kit *Red Fluorescence* | 559 | 635 | 38000 | 0.70001 |
Images
![Fluorescence images of intracellular lipid droplets in control (Left) and Oleic Acid treated HeLa cells (Right) using Cell Navigator® Lipid Droplets Fluorescence Assay Kit. HeLa cells were incubated with 300 uM of Oleic Acid for 24 hours to induce intracellular lipid droplets formation. After washing with PBS, the cells were labeled with 1X Nile Green™ and Hoechst 33342 (Cat#17533).](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fcell-navigator-fluorimetric-lipid-droplet-assay-kit-green-fluorescence%2Ffigure-for-cell-navigator-fluorimetric-lipid-droplet-assay-kit-green-fluorescence_BLMOb.jpg&w=3840&q=75)
Citations
Authors: Kishimura, Urara and Soeda, Shuhei and Ito, Daiki and Ueta, Yoko and Harada, Maki and Tanaka, Mai and Taniura, Hideo
Journal: Biochemical and Biophysical Research Communications (2024): 150124
Authors: Yin, Xuecui and Mi, Yang and Wang, Xiaohan and Li, Ya and Zhu, Xiaohui and Bukhari, Ihtisham and Wang, Qingde and Zheng, Pengyuan and Xue, Xia and Tang, Youcai
Journal: International Immunopharmacology (2024): 112177
Authors: Yang, Yuqi and Peng, Yue and Yu, Bin and Wang, Huiyan
Journal: Archives of Medical Research (2024): 102925
Authors: Kumar, Reetesh
Journal: bioRxiv (2020)
References
Authors: Nevo-Yassaf, I.; Lovelle, M.; Nahmias, Y.; Hirschberg, K.; Sklan, E. H.
Journal: Methods (2017)
Authors: Schneider, M. R.
Journal: Exp Cell Res (2016): 205-8
Authors: Wang, C. W., Lipid droplets, lipophagy
Journal: Biochim Biophys Acta (2016): 793-805
Authors: Makino, A.; Hullin-Matsuda, F.; Murate, M.; Abe, M.; Tomishige, N.; Fukuda, M.; Yamashita, S.; Fujimoto, T.; Vidal, H.; Lagarde, M.; Delton, I.; Kobayashi, T.
Journal: Mol Biol Cell (2016): 3293-3304
Authors: Takahashi, H.; Kutasy, B.; Friedmacher, F.; Takahashi, T.; Puri, P.
Journal: Pediatr Surg Int (2016): 155-60
Authors: Hashemi, H. F.; Goodman, J. M.
Journal: Curr Opin Cell Biol (2015): 119-24
Authors: Kochan, K.; Maslak, E.; Krafft, C.; Kostogrys, R.; Chlopicki, S.; Baranska, M., Raman spectroscopy analysis of lipid droplets content
Journal: J Biophotonics (2015): 597-609
Authors: Papadopoulos, C.; Orso, G.; Mancuso, G.; Herholz, M.; Gumeni, S.; Tadepalle, N.; Jungst, C.; Tzschichholz, A.; Schauss, A.; Honing, S.; Trifunovic, A.; Daga, A.; Rugarli, E. I.
Journal: PLoS Genet (2015): e1005149
Authors: Natarajan, S. K.; Rasineni, K.; Ganesan, M.; Feng, D.; McVicker, B. L.; McNiven, M. A.; Osna, N. A.; Mott, J. L.; Casey, C. A.; Kharb and a, K. K.
Journal: Curr Mol Pharmacol (2015)
Authors: Billecke, N.; Rago, G.; Bosma, M.; Eijkel, G.; Gemmink, A.; Leproux, P.; Huss, G.; Schrauwen, P.; Hesselink, M. K.; Bonn, M.; Parekh, S. H.
Journal: Histochem Cell Biol (2014): 263-73
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FAQ
What is the major type of lipid found in the cell membrane?
When using Cell Navigator® Fluorimetric Lipid Droplet Assay Kit which fluorescent dye should I use?
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