Cell Meter™ IX830 fixable viability dye
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Solvent | DMSO |
Excitation (nm) | 811 |
Emission (nm) | 822 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
Excitation (nm) 811 | Emission (nm) 822 |
Platform
Flow cytometer
Excitation | 634 laser or IR lasers |
Emission | 780/50 nm or IR filters |
Instrument specification(s) | APC/Cy7 or IR filters |
Example protocol
AT A GLANCE
Prepare samples in HHBS buffer (0.5 mL/assay).
Add Cell Meter™ IX830 to the cell suspension.
Stain the cells at room temperature for 20 - 60 minutes.
Wash the cells.
Fix the cells (optional).
Examine the sample with flow cytometer using 780/50 nm filter (APC-Cy7 channel) and/or IR filters.
Thaw at room temperature before starting the experiment.
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Add 200 µL of DMSO to the Cell Meter™ IX830 vial to make a 500X stock solution.
Note: Any unused Cell Meter™ IX830 stock solution should be divided into single-use aliquots and stored at -20°C. Avoid repeated freeze/thaw cycles.
SAMPLE EXPERIMENTAL PROTOCOL
Treat cells as desired.
Wash cells once with HHBS or the azide- and serum/protein-free buffer of your choice.
Resuspend cells at 5 - 10 × 10 6/mL in HHBS or in the azide- and serum/protein-free buffer of your choice.
Add 1 µL of 500X Cell Meter™ IX830 stock solution to 0.5 mL of cells/assay and mix it well.
Incubate at room temperature or 37°, 5% CO2 incubator for 20 - 60 minutes, protected from light.
Note: The optimal stain concentrations and incubation time should be experimentally determined for different cell lines.
Wash cells twice and resuspend cells with HHBS or the buffer of your choice.
Fix cells as desired (optional).
Analyze cells with a flow cytometer using 780/50 nm filter (APC-Cy7 channel) or IR filters.
Product Family
Name | Excitation (nm) | Emission (nm) |
Cell Meter™ BX520 fixable viability dye | 491 | 516 |
Cell Meter™ BX590 fixable viability dye | 492 | 579 |
Cell Meter™ BX650 fixable viability dye | 518 | 654 |
Cell Meter™ RX660 fixable viability dye | 649 | 664 |
Cell Meter™ RX700 fixable viability dye | 690 | 713 |
Cell Meter™ RX780 fixable viability dye | 629 | 767 |
Cell Meter™ VX450 fixable viability dye | 406 | 445 |
Cell Meter™ VX500 fixable viability dye | 433 | 498 |
Images
References
Authors: Xia, Fada and Chen, Yong and Jiang, Bo and Bai, Ning and Li, Xinying
Journal: Cancer cell international (2020): 49
Authors: Du Preez, C I and Gründemann, C and Mumbengegwi, D R and Huber, R
Journal: Journal of ethnopharmacology (2020): 112683
Authors: Persia, Fabio Andrés and Troncoso, Mariana Elizabeth and Rinaldini, Estefanía and Simirgiotis, Mario and Tapia, Alejandro and Bórquez, Jorge and Mackern-Oberti, Juan Pablo and Hapon, María Belén and Gamarra-Luques, Carlos
Journal: Heliyon (2020): e03353
Authors: da Cruz Filho, Iranildo José and da Silva Barros, Bárbara Rafaela and de Souza Aguiar, Lethícia Maria and Navarro, Claudia Daniele Carvalho and Ruas, Juliana Silveira and de Lorena, Virgínia Maria Barros and de Moraes Rocha, George Jackson and Vercesi, Aníbal Eugênio and de Melo, Cristiane Moutinho Lagos and Maior, Ana Maria Souto
Journal: International journal of biological macromolecules (2019): 1331-1339
Authors: Liu, Ran and Ni, Yangyue and Song, Jingwei and Xu, Zhipeng and Qiu, Jingfan and Wang, Lijuan and Zhu, Yuxiao and Huang, Yibing and Ji, Minjun and Chen, Yuxin
Journal: Parasite immunology (2019): e12619
Authors: Che, Song-Tian and Bie, Li and Li, Xu and Qi, Hui and Yu, Peng and Zuo, Ling
Journal: International journal of ophthalmology (2019): 1531-1538
Authors: Wen, Shi-Yuan and Chen, Yan-Yan and Deng, Chun-Miao and Zhang, Cui-Qiong and Jiang, Miao-Miao
Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (2019): 352-363
Authors: Lönnqvist, Susanna and Junker, Johan P E and Sedell, Maria and Nyman, Erika and Kratz, Gunnar
Journal: PloS one (2019): e0221878
Authors: Zimmermann-Klemd, Amy M and Konradi, Viktoria and Steinborn, Carmen and Ücker, Annekathrin and Falanga, Chiara Madlen and Woelfle, Ute and Huber, Roman and Jürgenliemk, Guido and Rajbhandari, Meena and Gründemann, Carsten
Journal: Journal of ethnopharmacology (2019): 415-423
Authors: Simchony, Hadar and Diamant, Diamant and Ram, Zvi and Volovitz, Ilan
Journal: The Israel Medical Association journal : IMAJ (2019): 503
Application notes
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
Annexin V