Cell Meter™ IX830 fixable viability dye

1. Prepare samples in HHBS buffer (0.5 mL/assay).

2. Add Cell Meter™ IX830 to the cell suspension.

3. Stain the cells at room temperature for 20 - 60 minutes.

4. Wash the cells.

5. Fix the cells (optional).

6. Examine the sample with flow cytometer using 780/50 nm filter (APC-Cy7 channel) and/or IR filters.

Thaw at room temperature before starting the experiment.

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles

1. Add 200 µL of DMSO to the Cell Meter™ IX830 vial to make a 500X stock solution.

   Note: Any unused Cell Meter™ IX830 stock solution should be divided into single-use aliquots and stored at -20°C. Avoid repeated freeze/thaw cycles.

1. Treat cells as desired.

2. Wash cells once with HHBS or the azide- and serum/protein-free buffer of your choice.

3. Resuspend cells at 5 - 10 × 10 ⁶/mL in HHBS or in the azide- and serum/protein-free buffer of your choice.

4. Add 1 µL of 500X Cell Meter™ IX830 stock solution to 0.5 mL of cells/assay and mix it well.

5. Incubate at room temperature or 37°, 5% CO₂ incubator for 20 - 60 minutes, protected from light.

   Note: The optimal stain concentrations and incubation time should be experimentally determined for different cell lines.

6. Wash cells twice and resuspend cells with HHBS or the buffer of your choice.

7. Fix cells as desired (optional).

8. Analyze cells with a flow cytometer using 780/50 nm filter (APC-Cy7 channel) or IR filters.