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Cell Meter™ Fixed Cell and Tissue TUNEL Apoptosis Assay Kit
Green Fluorescence
Cell Meter™ Fixed Cell and Tissue TUNEL Apoptosis Assay Kit provides a robust tool for conveniently detecting DNA fragmentation caused by apoptosis. The assay is a non-radioactive, simple, accurate and rapid method for monitoring apoptosis in. fixed cells and tissues via imaging DNA fragmentation. The TUNEL assay uses terminal deoxynucleotidyl transferase (TdT) to catalyze the incorporation of fluorescein-12-dUTP at the 3’-hydroxyl ends of the fragmented DNA. The fluorescein-labeled DNA is analyzed by fluorescence microscopy or flow cytometry (excitation at 488 nm with 530/30 nm emission filter). The kit can be used to detect apoptosis in fixed cells and formalin-fixed, paraffin-embedded tissue sections.
HeLa cells were fixed and treated with or without DNAse for 60 mins at 37 °C. The cells were then stained with Cell Meter™ TUNEL apoptosis assay kit. DNA strand breaks showed intense fluorescent staining in DNAse treated cells. The signal was acquired with fluorescence microscope using a FITC filter set.
HeLa cells were fixed and treated with or without DNAse for 60 mins at 37 °C. The cells were then stained with Cell Meter™ TUNEL apoptosis assay kit. DNA strand breaks showed intense fluorescent staining in DNAse treated cells. The signal was acquired with fluorescence microscope using a FITC filter set.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
2285125 Tests
Price
 
Spectral properties
Absorbance (nm)487
Correction factor (260 nm)0.32
Correction factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)
80000
1
Excitation (nm)498
Emission (nm)517
Quantum yield
0.7900
1
, 
0.95
2
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings
Flow cytometer
Excitation488 nm laser
Emission530/30 nm filter
Instrument specification(s)FITC channel
Fluorescence microscope
ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 8, 2024