Cell Meter™ Colorimetric WST-8 Cell Quantification Kit
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | ![]() ![]() |
Platform
Absorbance microplate reader
Absorbance | 460 nm |
Recommended plate | Clear bottom |
Example protocol
AT A GLANCE
Prepare cells in a 96-well plate (100 µL/well).
Add 10 µL of WST-8™ Solution to each well.
Incubate at 37°C for 1 - 4 hours.
Monitor absorbance at OD = 460 nm.
The WST-8™ Solution can be stable for up to one year if stored at 4°C and protected from light. Store it at <-20°C for longer storage.
CELL PREPARATION
For guidelines on cell sample preparation, please visit:
https://www.aatbio.com/resources/guides/cell-sample-preparation.html
SAMPLE EXPERIMENTAL PROTOCOL
- Plate 5000 to 10,000 cells/well in a tissue culture microplate with clear bottom.
Add test compounds into the cells and incubate for a desired period of time (such as 24, 48, or 96 hours) in a 37°C, 5% CO2 incubator. For blank wells (medium without the cells), add the same amount of test compounds. The suggested total volume is 100 µL for a 96-well plate or 50 µL for a 384-well plate.
Note: Each cell line should be evaluated on an individual basis to determine the optimal cell density for proliferation or cytotoxicity induction. For proliferation assays, use fewer cells; for cytotoxicity assays, use more cells to start with.
Add 10 µL/well (96-well plate) or 5 µL/well (384-well plate) of WST-8™ Solution to each well.
Incubate the plate at 37°C for 1 - 4 hours, protect from light.
Note: The incubation time could be from 30 minutes to overnight depending on the individual cell type and cell concentration used. Optimize the incubation time for each experiment.
- Monitor the absorbance increase with an absorbance plate reader at OD =460 nm.
Prepare cell culture in a tissue culture microplate with a clear bottom. The suggested total volume is 100 µL for a 96-well plate or 50 µL for a 384-well plate.
Note: We used serially diluted HeLa and Jurkat cell suspension in a clear bottom 96-well plate for the assay.
Add 10 µL/well (96-well plate) or 5 µL/well (384-well plate) of WST-8™ Solution to each well.
Incubate the plate at 37°C for 1 - 4 hours, protect from light.
Note: The incubation time could be from 30 minutes to overnight depending on the individual cell type and cell concentration used. Optimize the incubation time for each experiment.
- Monitor the absorbance increase with an absorbance plate reader at OD = 460 nm.
Images
![Cell number was determined with Cell Meter™ Colorimetric WST-8 Cell Quantification Kit. HeLa cells at 40 to 10,000 cells/well/100 µL were added in a clear bottom 96-well plate. The absorbance was measured at 460 nm using a SpectraMax reader (Molecular Devices).](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fcell-meter-colorimetric-wst-8-cell-quantification-kit%2Ffigure-for-cell-meter-colorimetric-wst-8-cell-quantification-kit_Ix3Os.png&w=3840&q=75)
![Cytotoxicity tests of HeLa cells in response to (A) SDS and (B) Staurosporine treatment were measured with Cell Meter™ Colorimetric WST-8 Cell Quantification Kit. HeLa cells at 10,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. Cells were treated with serially diluted SDS for 2 hours or Staurosporine for 4 hours. The absorbance was measured at 460 nm using a SpectraMax reader. ](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fcell-meter-colorimetric-wst-8-cell-quantification-kit%2Ffigure-for-cell-meter-colorimetric-wst-8-cell-quantification-kit_MxVMg.jpg&w=3840&q=75)
![Cell number was determined with Cell Meter™ Colorimetric WST-8 Cell Quantification Kit<strong>.</strong> Jurkat cells at 60 to 150,000 cells/well/100 µL were added in a clear bottom 96-well plate. The absorbance was measured at 460 nm using a SpectraMax reader (Molecular Devices).](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fcell-meter-colorimetric-wst-8-cell-quantification-kit%2Ffigure-for-cell-meter-colorimetric-wst-8-cell-quantification-kit_SK81h.png&w=3840&q=75)
![FGF10 increased the viability of primary mouse corneal epithelial cells. Mouse corneal epithelial cells were seeded in multi-well dishes, treated with FGF10 (30 ng/mL) or BSA (control), and cultured for 1, 3, and 7 days. Cells were processed for viability assay (tetrazolium salt, WST- 8). Dots represent the number of repeats (n = 9) for each condition/time. Statistical significance was assessed with unpaired t-tests. All bar plots are mean ± SD. *P < 0.05; ****P < 0.0001. Source: <b>Role of FGF10/FGFR2b Signaling in Homeostasis and Regeneration of Adult Lacrimal Gland and Corneal Epithelium Proliferation</b> by Findburgh <em>et.al.</em>, <em>Investigative Ophthalmology & Visual Science</em> Jan. 2023.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fcell-meter-colorimetric-wst-8-cell-quantification-kit%2Ffigure-for-cell-meter-colorimetric-wst-8-cell-quantification-kit_CLLfJ.png&w=3840&q=75)
Citations
Authors: Finburgh, Emma N and Mauduit, Olivier and Noguchi, Takako and Bu, Jennifer J and Abbas, Anser A and Hakim, Dominic F and Bellusci, Saverio and Meech, Robyn and Makarenkova, Helen P and Afshari, Natalie A
Journal: Investigative Ophthalmology \& Visual Science (2023): 21--21
Authors: Peng, Lilei and Ming, Yang and Zhang, Ling and Zhou, Jie and Xiang, Wei and Zeng, Shan and He, Haiping and Chen, Ligang
Journal: The FASEB Journal (2020): 5128--5143
Authors: Gao, Yong and Sun, Laisheng and Wu, Zicheng and Xuan, Chengmin and Zhang, Junxia and You, Yongping and Chen, Xincheng
Journal: Molecular Medicine Reports (2017)
References
Authors: Tiwari, K.; Wavdhane, M.; Haque, S.; Govender, T.; Kruger, H. G.; Mishra, M. K.; Ch and ra, R.; Tiwari, D.
Journal: Pharm Biol (2015): 849-54
Authors: Tsukatani, T.; Suenaga, H.; Shiga, M.; Noguchi, K.; Ishiyama, M.; Ezoe, T.; Matsumoto, K.
Journal: J Microbiol Methods (2012): 160-6
Authors: Stoddart, M. J.
Journal: Methods Mol Biol (2011): 21-5
Authors: Brady, A. J.; Kearney, P.; Tunney, M. M., Comparative evaluation of 2,3-bis [2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) and 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H-tetrazolium
Journal: J Microbiol Methods (2007): 305-11
Authors: Arai, M.; Kosuge, K.; Kawamoto, F.; Matsuoka, H.
Journal: Acta Med Okayama (2006): 127-34
Application notes
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
Annexin V