Cell Meter™ Cell Proliferation Assay Kit

1. Prepare the cell samples and treat cells as desired

2. Remove cell culture medium

3. Add dye working solution

4. Incubate for 10 minutes

5. Analyze with fluorescence microplate reader using Ex/Em = 490/525 nm

Important: Allow all the components to warm to room temperature before opening the vials. Because this reagent binds to nucleic acids, it should be treated as a potential mutagen and handled with appropriate care. The DMSO stock solution should be handled with particular caution as DMSO is known to facilitate the entry of organic molecules into tissues. 

Add 25 µL of Nuclear Green™ LCS1 to 10 mL of Reaction Buffer and mix well.

Note: 10 mL volume is sufficient for 100 tests. For best results, the Nuclear Green™ LCS1 dye working solution should be used promptly and in its entirety. 

Note: Store any unused Nuclear Green™ LCS1 (Component A) at -20 °C.

The following protocol can be used for guidelines.

1. Prepare the cell samples and treat cells as desired.

2. Remove the cell culture medium and wash twice with PBS.

   Note: Removal of cell culture medium is necessary since it may interfere with the fluorescence of Nuclear Green™ LCS1.

3. Optional: After washing with PBS, experiments involving multiple time points and/or samples can be stored at -80 °C for a few days or proceed to step 4.

4. Add 100 µL of Nuclear Green™ LCS1 dye working solution and incubate for 10 to 60 minutes at RT, protected from light.

5. Measure the sample fluorescence using a fluorescence microplate reader with Ex/Em = 490/525 nm (Cutoff = 515 nm).