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Cal-590™-Dextran Conjugate *MW 3,000*

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cells may be physically loaded with the cell-impermeant salt forms of these dextran-conjugated calcium indicators using patch pipette or microinjection. The fluorescence signal from these cells is measured using fluorescence microscopy. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-590 dextran conjugates might be a better choice than other red fluorescent dextrtan conjugates due to its higher fluorescence quantum yield and larger fluorescence enhancement by calcium.

Spectrum

Product family

NameExcitation (nm)Emission (nm)
Cal-590L® Dextran Conjugate *MW 10,000*569590
Cal-630™-Dextran Conjugate *MW 3,000*609626
Cal-630™-Dextran Conjugate *MW 10,000*609626
Cal-520®-Dextran Conjugate *MW 3,000*492515
Cal-520®-Dextran Conjugate *MW 10,000*492515
Cal-670™-Dextran Conjugate *MW 3,000*667680
Cal-670™-Dextran Conjugate *MW 10,000*667680
Cal-770™-Dextran Conjugate *MW 3,000*758783
Cal-770™-Dextran Conjugate *MW 10,000*758783

Citations

View all 6 citations: Citation Explorer
Zebrafish oxytocin neurons drive nocifensive behavior via brainstem premotor targets
Authors: Wee, Caroline L and Nikitchenko, Maxim and Wang, Wei-Chun and Luks-Morgan, Sasha J and Song, Erin and Gagnon, James A and Randlett, Owen and Bianco, Isaac H and Lacoste, Alix MB and Glushenkova, Elena and others,
Journal: Nature neuroscience (2019): 1
Behavioral role of the reciprocal inhibition between a pair of Mauthner cells during fast escapes in zebrafish
Authors: Shimazaki, Takashi and Tanimoto, Masashi and Oda, Yoichi and Higashijima, Shin-ichi
Journal: Journal of Neuroscience (2018): 1964--18
Ca 2+ signals initiate at immobile IP 3 receptors adjacent to ER-plasma membrane junctions
Authors: Thillaiappan, Nagendra Babu and Chavda, Alap P and Tovey, Stephen C and Prole, David L and Taylor, Colin W
Journal: Nature Communications (2017): 1505
α V β 3 Integrin regulates astrocyte reactivity
Authors: Lagos-Cabré, Raúl and Alvarez, Alvaro and Kong, Milene and Burgos-Bravo, Francesca and Cárdenas, Areli and Rojas-Mancilla, Edgardo and Pérez-Nunez, Ramón and Herrera-Molina, Rodrigo and Rojas, Fabiola and Schneider, Pascal and others, undefined
Journal: Journal of Neuroinflammation (2017): 194
In vivo deep two-photon imaging of neural circuits with the fluorescent Ca2+ indicator Cal-590
Authors: Tischbirek, Carsten H and Birkner, Antje and Konnerth, Arthur
Journal: The Journal of Physiology (2016)

References

View all 32 references: Citation Explorer
High Affinity Mannotetraose as an Alternative to Dextran in ConA Based Fluorescent Affinity Glucose Assay Due to Improved FRET Efficiency
Authors: Locke, A. K.; Cummins, B. M.; Cote, G. L.
Journal: ACS Sens (2016): 584-590
Synthesis and Cell Imaging of a Near-Infrared Fluorescent Magnetic "CdHgTe-Dextran-Magnetic Layered Double Hydroxide-Fluorouracil" Composite
Authors: Jin, X.; Zhang, M.; Gou, G.; Ren, J.
Journal: J Pharm Sci (2016): 1751-61
The effect of the size of fluorescent dextran on its endocytic pathway
Authors: Li, L.; Wan, T.; Wan, M.; Liu, B.; Cheng, R.; Zhang, R.
Journal: Cell Biol Int (2015): 531-9
Visualizing Lysosomal Membrane Permeabilization by Fluorescent Dextran Release
Authors: Ellegaard, A. M.; Jaattela, M.; Nyl and sted, J.
Journal: Cold Spring Harb Protoc (2015): 900-3
Characterization of biomimetic calcium phosphate labeled with fluorescent dextran for quantification of osteoclastic activity
Authors: Maria, S. M.; Prukner, C.; Sheikh, Z.; Muller, F. A.; Komarova, S. V.; Barralet, J. E.
Journal: Acta Biomater (2015): 140-6
Page updated on November 21, 2024

Ordering information

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Unit size
Catalog Number20508
Quantity
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Physical properties

Molecular weight

~4000

Solvent

Water

Spectral properties

Extinction coefficient (cm -1 M -1)

78000

Excitation (nm)

563

Emission (nm)

584

Quantum yield

0.621

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
ATP-stimulated calcium response of endogenous P2Y receptor in CHO-K1 cells incubated with Cal-590&reg; AM (red) or Rhod-2 AM (blue)under the same conditions. CHO-K1 cells were seeded overnight at 50,000 cells per 100 &micro;L per well in a 96-well black wall/clear bottom Costar plate. 100 &micro;L of 5 &micro;g/mL Cal-590&reg; AM or Rhod-2 AM with 2.5 mM probenecid was added into the cells, and the cells were incubated at 37 &deg;C for 1 hour. ATP (50 &micro;L/well) was added by FlexStation (Molecular Devices) to achieve the final indicated concentrations.
ATP-stimulated calcium response of endogenous P2Y receptor in CHO-K1 cells incubated with Cal-590&reg; AM (red) or Rhod-2 AM (blue)under the same conditions. CHO-K1 cells were seeded overnight at 50,000 cells per 100 &micro;L per well in a 96-well black wall/clear bottom Costar plate. 100 &micro;L of 5 &micro;g/mL Cal-590&reg; AM or Rhod-2 AM with 2.5 mM probenecid was added into the cells, and the cells were incubated at 37 &deg;C for 1 hour. ATP (50 &micro;L/well) was added by FlexStation (Molecular Devices) to achieve the final indicated concentrations.
ATP-stimulated calcium response of endogenous P2Y receptor in CHO-K1 cells incubated with Cal-590&reg; AM (red) or Rhod-2 AM (blue)under the same conditions. CHO-K1 cells were seeded overnight at 50,000 cells per 100 &micro;L per well in a 96-well black wall/clear bottom Costar plate. 100 &micro;L of 5 &micro;g/mL Cal-590&reg; AM or Rhod-2 AM with 2.5 mM probenecid was added into the cells, and the cells were incubated at 37 &deg;C for 1 hour. ATP (50 &micro;L/well) was added by FlexStation (Molecular Devices) to achieve the final indicated concentrations.
The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-590 dextran conjugates might be a better choice than other red fluorescent dextrtan conjugates due to its higher fluorescence quantum yield and larger fluorescence enhancement by calcium.

Documents

pdfSafety data sheet
pdfProduct protocol
pdfCertificate of analysis
Calcium Indicators
Dextran Conjugates
Multi-Photon Microscopy

Alternative formats

Cal-590™-Dextran Conjugate *MW 10,000*
Cal-590™ AM
Cal-590™, sodium salt
Cal-590™, potassium salt