Cal-520®-Dextran Conjugate *MW 3,000*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | ~4000 |
Solvent | Water |
Spectral properties
Excitation (nm) | 492 |
Emission (nm) | 515 |
Quantum yield | 0.751 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Alternative formats
Cal-520®-Dextran Conjugate *MW 10,000* |
Overview | ![]() ![]() |
Molecular weight ~4000 | Excitation (nm) 492 | Emission (nm) 515 | Quantum yield 0.751 |
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cells may be physically loaded with the cell-impermeant salt forms of these dextran-conjugated calcium indicators using patch pipette or microinjection. The fluorescence signal from these cells is measured using fluorescence microscopy. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-520 dextran conjugates might be the best choice due to their high fluorescence quantum yield and large fluorescence enhancement by calcium. Compared to Oregon Green BAPTA-1 dextran, Cal-520 dextran exhibits much lower background and much larger calcium-induced fluorescence enhancement.
Spectrum
Open in Advanced Spectrum Viewer
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Fcal_520.png&w=2048&q=50)
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Fcal_520.png&w=2048&q=50)
Spectral properties
Excitation (nm) | 492 |
Emission (nm) | 515 |
Quantum yield | 0.751 |
Product Family
Name | Excitation (nm) | Emission (nm) | Quantum yield |
Cal-520®-Biotin Conjugate | 492 | 515 | 0.751 |
Cal-520®-Biocytin Conjugate | 492 | 515 | 0.751 |
Cal-590™-Dextran Conjugate *MW 3,000* | 563 | 584 | 0.621 |
Cal-590™-Dextran Conjugate *MW 10,000* | 563 | 584 | 0.621 |
Cal-630™-Dextran Conjugate *MW 3,000* | 609 | 626 | 0.371 |
Cal-630™-Dextran Conjugate *MW 10,000* | 609 | 626 | 0.371 |
Cal-670™-Dextran Conjugate *MW 3,000* | 667 | 680 | - |
Cal-670™-Dextran Conjugate *MW 10,000* | 667 | 680 | - |
Cal-770™-Dextran Conjugate *MW 3,000* | 758 | 783 | - |
Show More (2) |
Images
Citations
View all 99 citations: Citation Explorer
High-Speed Neural Imaging with Synaptic Resolution: Bessel Focus Scanning Two-Photon Microscopy and Optical-Sectioning Widefield Microscopy
Authors: Meng, Guanghan and Zhang, Qinrong and Ji, Na
Journal: (2023): 293--329
Authors: Meng, Guanghan and Zhang, Qinrong and Ji, Na
Journal: (2023): 293--329
Astrocytic glutamate transporters reduce the neuronal and physiological influence of metabotropic glutamate receptors in nucleus tractus solitarii
Authors: Martinez, Diana and Rogers, Richard C and Hermann, Gerlinda E and Hasser, Eileen M and Kline, David D
Journal: American Journal of Physiology-Regulatory, Integrative and Comparative Physiology (2020): R545--R564
Authors: Martinez, Diana and Rogers, Richard C and Hermann, Gerlinda E and Hasser, Eileen M and Kline, David D
Journal: American Journal of Physiology-Regulatory, Integrative and Comparative Physiology (2020): R545--R564
Information processing in the olfactory system of different amphibian species
Authors: Weiss, Lukas
Journal: (2020)
Authors: Weiss, Lukas
Journal: (2020)
Phosphatidylinositol 3, 5-Bisphosphate regulates Ca2+ Transport During Yeast Vacuolar Fusion through Activation of the Ca2+ ATPase Pmc1
Authors: Fratti, Rutilio A and Miner, Gregory E and Sullivan, Katherine D and Guo, Annie and Starr, Matthew L and Ellis, Ez and Jones, Br and on, undefined
Journal: bioRxiv (2019): 533067
Authors: Fratti, Rutilio A and Miner, Gregory E and Sullivan, Katherine D and Guo, Annie and Starr, Matthew L and Ellis, Ez and Jones, Br and on, undefined
Journal: bioRxiv (2019): 533067
Response patterns and neurogenesis of the olfactory bulb of Xenopus laevis during metamorphosis
Authors: ARIAS, PAOLA LUZ ERENDIDA SEGOVIANO and others,
Journal: (2018)
Authors: ARIAS, PAOLA LUZ ERENDIDA SEGOVIANO and others,
Journal: (2018)
Mitochondrial control of calcium signaling in native endothelium
Authors: Wilson, Calum and Lee, Matthew and McCarron, John G
Journal: The FASEB Journal (2017): 1080--7
Authors: Wilson, Calum and Lee, Matthew and McCarron, John G
Journal: The FASEB Journal (2017): 1080--7
A Critical Period for the Rapid Modification of Synaptic Properties at the VPm Relay Synapse
Authors: Pan, Libiao and Yang, Junhua and Yang, Qian and Wang, Xiaomeng and Zhu, Liya and Liu, Yali and Lou, Huifang and Xu, Chou and Shen, Ying and Wang, Hao
Journal: Frontiers in molecular neuroscience (2017)
Authors: Pan, Libiao and Yang, Junhua and Yang, Qian and Wang, Xiaomeng and Zhu, Liya and Liu, Yali and Lou, Huifang and Xu, Chou and Shen, Ying and Wang, Hao
Journal: Frontiers in molecular neuroscience (2017)
Calcium Imaging Across Large Areas of Intact Vascular Endothelium Reveals Stimulus-Specific Sensory Cells
Authors: Lee, Matthew D and Wilson, Calum and McCarron, John G
Journal: The FASEB Journal (2017): 1005--8
Authors: Lee, Matthew D and Wilson, Calum and McCarron, John G
Journal: The FASEB Journal (2017): 1005--8
Characterization of postsynaptic calcium signals in the pyramidal neurons of anterior cingulate cortex
Authors: Li, Xu-Hui and Song, Qian and Chen, Tao and Zhuo, Min
Journal: Molecular Pain (2017): 1744806917719847
Authors: Li, Xu-Hui and Song, Qian and Chen, Tao and Zhuo, Min
Journal: Molecular Pain (2017): 1744806917719847
Prenatal thalamic waves regulate cortical area size prior to sensory processing
Authors: Moreno-Juan, Verónica and Filipchuk, Anton and Antón-Bolanos, Noelia and Mezzera, Cecilia and Gezelius, Henrik and Andrés, Belen and Rodríguez-Malmierca, Luis and Susín, Rafael and Schaad, Olivier and Iwasato, Takuji and others, undefined
Journal: Nature Communications (2017): 14172
Authors: Moreno-Juan, Verónica and Filipchuk, Anton and Antón-Bolanos, Noelia and Mezzera, Cecilia and Gezelius, Henrik and Andrés, Belen and Rodríguez-Malmierca, Luis and Susín, Rafael and Schaad, Olivier and Iwasato, Takuji and others, undefined
Journal: Nature Communications (2017): 14172
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
FAQ
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?