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Cal-520L®-Dextran Conjugate *MW 10,000*
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cells may be physically loaded with the cell-impermeant dextran-conjugated calcium indicators using patch pipette or microinjection. The fluorescence signal from these cells is measured using fluorescence microscopy. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-520 dextran conjugates might be the best choice due to their high fluorescence quantum yield and large fluorescence enhancement by calcium. This Cal-520L dextran exhibits low affinity to calcium ion than Cal-520 dextran, and optimized for monitoring high level calcium ion.
Product family
| Name | Excitation (nm) | Emission (nm) | Quantum yield |
| Cal-520®-Dextran Conjugate *MW 3,000* | 492 | 515 | 0.751 |
| Cal-520®-Dextran Conjugate *MW 10,000* | 492 | 515 | 0.751 |
| Cal-590™-Dextran Conjugate *MW 3,000* | 563 | 584 | 0.621 |
| Cal-590™-Dextran Conjugate *MW 10,000* | 563 | 584 | 0.621 |
| Cal-630™-Dextran Conjugate *MW 3,000* | 609 | 626 | 0.371 |
| Cal-630™-Dextran Conjugate *MW 10,000* | 609 | 626 | 0.371 |
| Cal-670™-Dextran Conjugate *MW 3,000* | 667 | 680 | - |
| Cal-670™-Dextran Conjugate *MW 10,000* | 667 | 680 | - |
| Cal-770™-Dextran Conjugate *MW 3,000* | 758 | 783 | - |
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Citations
View all 52 citations: Citation Explorer
Molecular dynamics simulations reveal the influence of dextran sulfate in nanoparticle formation with calcium alginate to encapsulate insulin
Authors: Nadvorny, D., Soares-Sobrinho, J. L., de La Roca Soares, M. F., Ribeiro, A. J., Veiga, F., Seabra, G. M.
Journal: J Biomol Struct Dyn (2018): 1255-1260
Authors: Nadvorny, D., Soares-Sobrinho, J. L., de La Roca Soares, M. F., Ribeiro, A. J., Veiga, F., Seabra, G. M.
Journal: J Biomol Struct Dyn (2018): 1255-1260
Sustained Release of Minocycline From Minocycline-Calcium-Dextran Sulfate Complex Microparticles for Periodontitis Treatment
Authors: Wu, L., Chen, W., Li, F., Morrow, B. R., Garcia-Godoy, F., Hong, L.
Journal: J Pharm Sci (2018): 3134-3142
Authors: Wu, L., Chen, W., Li, F., Morrow, B. R., Garcia-Godoy, F., Hong, L.
Journal: J Pharm Sci (2018): 3134-3142
Calcium/Ask1/MKK7/JNK2/c-Src signalling cascade mediates disruption of intestinal epithelial tight junctions by dextran sulfate sodium
Authors: Samak, G., Chaudhry, K. K., Gangwar, R., Narayanan, D., Jaggar, J. H., Rao, R.
Journal: Biochem J (2015): 503-15
Authors: Samak, G., Chaudhry, K. K., Gangwar, R., Narayanan, D., Jaggar, J. H., Rao, R.
Journal: Biochem J (2015): 503-15
Monitoring of single-cell responses in the optic tectum of adult zebrafish with dextran-coupled calcium dyes delivered via local electroporation
Authors: Kassing, V., Engelmann, J., Kurtz, R.
Journal: PLoS One (2013): e62846
Authors: Kassing, V., Engelmann, J., Kurtz, R.
Journal: PLoS One (2013): e62846
Forward-filling of dextran-conjugated indicators for calcium imaging at the Drosophila larval neuromuscular junction
Authors: Macleod, G. T.
Journal: Cold Spring Harb Protoc (2012): 791-6
Authors: Macleod, G. T.
Journal: Cold Spring Harb Protoc (2012): 791-6
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