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Buccutite™ FOL scavenger
Our Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield. Our method uses one pair of crosslinkers: Buccutite™ MTA and Buccutite™ FOL. MTA is added to one molecule, while FOL is added to another molecule. The cross-linking reaction is initiated by mixing Molecule-1-Buccutite ™ MTA and Molecule-2-Buccutite ™ FOL. This crosslinking reaction occurs under extremely mild and neutral conditions without any catalyst required. It is robust and efficient. Many of our customer have requested us to offer the stand-alone Buccutite™ MTA and Buccutite™ FOL reagents to expand the application of Buccutite™crosslinking technology. This Buccutite™ FOL reagent is used to eliminate unreacted FOL groups for the crosslinked product if desired. For the vast majority of applications, there is no need to block the unreacted FOL groups from the final crosslinked product.
Example protocol
AT A GLANCE
- Prepare Buccutite™ FOL scavenger stock solution.
- Add 1 uL /100 uL reaction mixture.
- Incubate at room temperature for 30~60 minutes.
PREPARATION OF STOCK SOLUTION
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.
Add 200 uL DMSO to Buccutite™ FOL scavenger vial to prepare 10 mM stock solution. Note: The Buccutite™ FOL scavenger stock solution should be stored at -20 °C after preparation and stable for 2 months if avoid repeated freeze-thaw cycles.
SAMPLE EXPERIMENTAL PROTOCOL
- Add 1 uL /100 uL reaction mixture so the final concentration of Buccutite™ FOL scavenger in reaction mixture is 100 uM.
- Incubate at room temperature for 30~60 minutes. Note: 1 uL Buccutite™ FOL scavengerstock solution is enough to scavenge FOL groups in solution when the concentration is lower than 100 uM. If there is higher FOL groups in the mixture, the volume needs to be adjusted to scavenge all the FOL groups.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Buccutite™ FOL scavenger to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 248.447 µL | 1.242 mL | 2.484 mL | 12.422 mL | 24.845 mL |
| 5 mM | 49.689 µL | 248.447 µL | 496.894 µL | 2.484 mL | 4.969 mL |
| 10 mM | 24.845 µL | 124.224 µL | 248.447 µL | 1.242 mL | 2.484 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Page updated on November 20, 2024
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