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Biotin-xtraFluor™ Violet 420 Conjugate

Product key features

  • Optimized to be maximally excited at 405 nm (Violet laser)
  • Optimized for multicolor flow cytometry applications
  • Spectrally compatible with the filter set of BV421
  • Probably the brightest violet laser-excitable biotin conjugate

Product description

Biotin-xtraFluor™ Violet 420 Conjugate might be the brightest biotin dye conjugate commercially available. It can be conveniently used for detecting avidin-tagged biomolecules. XV420 is an extremely bright violet laser-excitable fluorophore. With a maximum excitation well matched the 405 nm violet laser and an emission peak at ~420 nm, XV420 can be conveniently used to replace BD Horizon Brilliant™ Violet 421 (BV421). It can be used on flow cytometers equipped with a violet laser and appropriate filters for large multicolor flow cytometry panels. It is a brighter alternative to Pacific Blue (ThermoFisher) and BD Horizon™ V450 (BD Biosciences). XV420 enables improved resolution for dim population identification. It is particularly useful in multicolor applications where it can be used to better resolve dim populations. XV420 dye offers new options for multicolor panel design through the use of a violet laser. Additionally, the dye’s spillover properties, stability under light and in standard buffers, and compatibility with blood collection tubes offer ease-of-use for a range of applications.

Spectrum

References

View all 49 references: Citation Explorer
Profiling of Surface Protein Epitopes on Viral Particles by Multiplex Dual-Reporter Strategy.
Authors: Sahi, Maryam and Andersson, Sarah and Mattson, Cecilia and Dale, Matilda and Kagiolglou, Sofia and Hofström, Camilla and Persson, Helena and Klingström, Jonas and Chiodi, Francesca and Fredolini, Claudia
Journal: Journal of visualized experiments : JoVE (2024)
Effects of solid retention time and exposure mode to electric current on Remazol Brilliant Violet removal in an electro-membrane bioreactor.
Authors: Belli, Tiago José and Bassin, João Paulo and de Sousa Vidal, Carlos Magno and Hassemer, Maria Eliza Nagel and Rodrigues, Caroline and Lapolli, Flávio Rubens
Journal: Environmental science and pollution research international (2023): 58412-58427
Sonophotocatalytic Degradation of Fast Yellow AB and Remazol Brilliant Violet-5R by Using Ag-Impregnated ZnO as a Photocatalyst.
Authors: Aziz, Nosheen and Rasool, Shagufta and Ullah, Toheed and Khitab, Fatima and Halim, Sobia Ahsan and Bawazeer, Sami and Khan, Ajmal and Al-Harrasi, Ahmed
Journal: ACS omega (2023): 18509-18515
Performance of treatment schemes comprising chromium-hydrogen peroxide-based advanced oxidation process for textile wastewater.
Authors: Mondal, Prasenjit and Mukherji, Suparna and Garg, Anurag
Journal: Environmental science and pollution research international (2022): 88089-88100
Comparative study and dyeing performance of as-synthesized azo heterocyclic monomeric, polymeric, and commercial disperse dyes.
Authors: Abdullahi, Shehu Sa'ad and Musa, Haruna and Habibu, Shehu and Birniwa, Abdullahi Haruna and Mohammad, Rania Edrees Adam
Journal: Turkish journal of chemistry (2022): 1841-1852
Page updated on April 15, 2025

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Catalog Number70804
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Physical properties

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.067

Extinction coefficient (cm -1 M -1)

3,000,000

Excitation (nm)

407

Emission (nm)

424

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure