ATTO 532 PEG3 azide

• Ex/Em: 531/552 nm
• Extinction coefficient: 115,000 cm^-1M^-1
• Reactive group: azide
• Click Chemistry: enables efficient, bioorthogonal labeling of alkyne-functionalized biomolecules
• High Quantum Yield & Photostability: ensures intense fluorescence signals for reliable detection and imaging
• Excellent Hydrophilicity: Prevents aggregation and enhances signal clarity in live-cell applications
• Advanced Imaging Performance: Suitable for single-molecule detection and super-resolution techniques, including SIM and STED

ATTO 532 is a rhodamine-based fluorescent dye notable for its high molar absorptivity and fluorescence quantum yield (0.90), providing robust signal intensity in fluorescence applications. Its combination of photostability, aqueous solubility, and sufficient Stokes shift makes it suitable for single-molecule detection and high-resolution microscopy techniques, including SIM and STED microscopy. ATTO 532 is also effective in flow cytometry, FISH, and various biological assays, offering flexibility for diverse fluorescence-based experimental protocols. The dye is optimally excited within the 515-545 nm range, with a frequency-doubled Nd:YAG laser at 532 nm serving as an ideal excitation source.

The azide derivative of ATTO 532 is widely used for labeling terminal alkynes on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with alkyne-containing molecules and strain-promoted alkyne-azide cycloaddition (SPAAC) with DBCO- or BCN-containing molecules.