ATTO 488 PEG3 azide

• Ex/Em: 499/520 nm
• Extinction coefficient: 90,000 cm^-1M^-1
• Reactive group: azide
• Click Chemistry Conjugation: Efficiently labels terminal alkynes via CuAAC and copper-free SPAAC, ideal for peptides, antibodies, and biomolecules
• Superior Brightness & Stability: Delivers robust, steady fluorescence for sensitive applications
• Hydrophilic PEG3 Spacer: Improves solubility and minimizes steric hindrance for enhanced biocompatibility

ATTO 488 is a hydrophilic, rhodamine-based fluorescent dye that exhibits enhanced water solubility due to the incorporation of a PEG3 spacer. It is characterized by strong absorption, a high fluorescence quantum yield, and exceptional photostability, making it highly suitable for advanced fluorescence imaging techniques. The dye exhibits optimal excitation within the 480-515 nm wavelength range, aligning precisely with the 488 nm emission line of the Argon-Ion laser. ATTO 488 is particularly effective for single-molecule detection and super-resolution microscopy methods such as PALM, dSTORM, and STED. Moreover, it is well-suited for flow cytometry (FACS), fluorescence in situ hybridization (FISH), and other bioanalytical applications.

The PEG3-azide derivative of ATTO 488 is widely used for labeling terminal alkynes on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with alkyne-containing molecules and strain-promoted alkyne-azide cycloaddition (SPAAC) with DBCO- or BCN-containing molecules.