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AAT Bioquest

APC/XFD750 Anti-mouse CD197 Antibody *4B12, XFD750 Same Structure to Alexa Fluor™ 750*

4B12 is an anti-mouse monoclonal antibody that is specific for the CD197 antigen. CD197 (sometimes called BLR2, CCR7, EBI1 or CMKBR7) is a glycoprotein that is expressed on the surface of cells like B cells, T cells and dendritic cells. CD197 is associated with a variety of biologically interesting macromolecules/ligands, for example, CCL19/ECL and CCL21. CD197 is a relatively rare antibody target, with fewer than 500 publications in the last decade. Even still, CD197 is essential for immunology research, commonly serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to APC/XFD750 (ex/em = 756/785 nm). XFD750 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 750 (Alexa Fluor® is the trademark of ThermoFisher).

Spectrum

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd197 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on January 24, 2025

Ordering information

Price
Unit size
25 tests
100 tests
500 tests
Catalog Number
119701D0119701D1119701D2
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Purchase orderSend to sales@aatbio.com
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Antibody properties

Other namesCCR7; BLR2; EBI1; CMKBR7
Clone4B12
Hostrat
IsotypeRat IgG2a kappa
Reactivitymouse
Application

Spectral properties

Extinction coefficient (cm -1 M -1)

700000

Excitation (nm)

651

Emission (nm)

785

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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