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AAT Bioquest

APC/XFD750 Anti-human CD4 Antibody *SK3, XFD750 Same Structure to Alexa Fluor™ 750*

SK3 is an anti-human monoclonal antibody that recognizes the CD4 antigen. CD4 (alternatively called T4 or Leu3a) is a 55 kD member of the Ig superfamily that is located on the surface of cells such as macrophages, T cells and granulocytes. CD4 has been associated with important biological processes such as membrane organization, specifically fusion of virus membrane with host plasma membrane. Furthermore, it is involved with vital cellular pathways, for example, the T cell receptor signaling pathway, enzyme linked receptor protein signaling pathway and cytokine-mediated signaling pathway. In some organisms, CD4 acts to positively regulate peptidyl-tyrosine phosphorylation, is an enhancer of transcription, DNA-templated and is a positive regulator of ERK1 and ERK2 cascade. From a research standpoint, it is of biological interest due to its association with essential macromolecules/ligands such as MHC Class II, gp120, IL-16 and Lck. CD4 is a very popular antibody target, with over 180000 publications in the last decade. CD4 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography and conjugated to APC/XFD750 (ex/em = 756/785 nm). XFD750 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 750 (Alexa Fluor® is the trademark of ThermoFisher).

Spectrum

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd4 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on November 20, 2024

Ordering information

Price
Unit size
25 tests
100 tests
500 tests
Catalog Number
100421E0100421E1100421E2
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
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Antibody properties

Other namesLeu-3; T4
CloneSK3
Hostmouse
IsotypeMouse IgG1, κ
Reactivityhuman
Application

Spectral properties

Extinction coefficient (cm -1 M -1)

700000

Excitation (nm)

651

Emission (nm)

785

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
Flow cytometry analysis of whole blood stained with APC-XFD750 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-XFD750 specific R7-A channel. XFD750 is the same structure as Alexa Fluor® 750.
Flow cytometry analysis of whole blood stained with APC-XFD750 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-XFD750 specific R7-A channel. XFD750 is the same structure as Alexa Fluor® 750.
Flow cytometry analysis of whole blood stained with APC-XFD750 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-XFD750 specific R7-A channel. XFD750 is the same structure as Alexa Fluor® 750.
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