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AAT Bioquest

Anti-Prolactin (PRL) antibody *Mouse anti-human, monoclonal IgG2b*

Prolactin (PRL), also known as luteotropic hormone, is a protein secreted from the pituitary gland. It plays a vital role in metabolism, immune system regulation and pancreatic development, but is best known for its role in producing milk in mammals. In normal individuals, PRL concentrations increase in response to physiologic stimuli such as eating, mating, exercise, stress and hypoglycemia. PRL levels are also elevated during pregnancy, lactation, postpartum and in newborn infants. In clinical biology, prolactin tests are used in the diagnosis of prolactinoma, a prolactin-producing tumor of the pituitary gland. Anti-PRL monoclonal antibodies are highly sensitive for the detection of human purified prolactin. They can be used for a broad range of immunoassays such as chemiluminescent immunoassays (CLIA) and in the development of highly sensitive sandwich immunoassays.

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of Anti-Prolactin (PRL) antibody *Mouse anti-human, monoclonal IgG2b* with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on October 31, 2024

Ordering information

Price
Unit size
50 ug
1 mg
Catalog Number
Quantity
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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ShippingStandard overnight for United States, inquire for international
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Antibody properties

Other names
Hostmouse
Reactivityhuman
ApplicationELISA; CLIA

Storage, safety and handling

Intended useResearch Use Only (RUO)
Clinical comparison of AAT Bioquest’s prolactin immunoassays and the Access Prolactin assay from Beckman Coulter: 80 clinical blood samples were separately tested using MAb combination A and B on AAT Bioquest’s CLIA platform and compared to a diagnostic kit from Beckman Coulter. Data from this study were analyzed using the Passing-Bablok regression method and are summarized in the following scatter plot. Results show good agreement between AAT Bioquest’s immunoassays and comparison assays.
Clinical comparison of AAT Bioquest’s prolactin immunoassays and the Access Prolactin assay from Beckman Coulter: 80 clinical blood samples were separately tested using MAb combination A and B on AAT Bioquest’s CLIA platform and compared to a diagnostic kit from Beckman Coulter. Data from this study were analyzed using the Passing-Bablok regression method and are summarized in the following scatter plot. Results show good agreement between AAT Bioquest’s immunoassays and comparison assays.
Clinical comparison of AAT Bioquest’s prolactin immunoassays and the Access Prolactin assay from Beckman Coulter: 80 clinical blood samples were separately tested using MAb combination A and B on AAT Bioquest’s CLIA platform and compared to a diagnostic kit from Beckman Coulter. Data from this study were analyzed using the Passing-Bablok regression method and are summarized in the following scatter plot. Results show good agreement between AAT Bioquest’s immunoassays and comparison assays.
Calibration curves for prolactin in sandwich chemiluminescence immunoassay (CLIA). All monoclonal antibodies were tested in pairs as capture and detection antibodies to determine the best two-site MAb combinations for the development of a quantitative sandwich immunoassay. Detection antibodies were labeled with horseradish peroxidase (HRP). The best selected MAb combinations for quantification of human prolactin are (capture-detection): MAb combination A: Cat# V100160 (Clone 1) - Cat# V100160 (Clone 2)ï¼›MAb combination B: Cat# V100160 (Clone 1) - Cat# V100160 (Clone 3).