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AAT Bioquest

Anti-Procalcitonin (PCT) antibody *Mouse anti-human, monoclonal IgG2b*

Procalcitonin (PCT) is a peptide precursor of calcitonin and is composed of 116 amino acids. It is produced by the C cells of the thyroid and by the neuroendocrine cells of the lung and the intestine. Under normal conditions, the level of PCT in the blood of healthy individuals is very low (< .05 ng/mL) with a half-life of 25 to 30 hours in serum. In response to a pro-inflammatory stimulus, such as a pathogen, the level of PCT may increase as high as 100 μg/mL making it a suitable biomarker of bacterial infection. In clinical biology, PCT has shown to be a useful tool in the diagnosis of sepsis. Anti-PCT monoclonal antibodies are highly specific for the detection of human procalcitonin. They can be used for a broad range of immunoassays such as chemiluminescent immunoassays (CLIA).

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of Anti-Procalcitonin (PCT) antibody *Mouse anti-human, monoclonal IgG2b* with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on October 31, 2024

Ordering information

Price
Unit size
50 ug
1 mg
Catalog Number
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
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Antibody properties

Other names
Hostmouse
Reactivityhuman
ApplicationELISA; CLIA

Physical properties

Solvent

Water

Storage, safety and handling

Intended useResearch Use Only (RUO)

Storage

Freeze (< -15 °C)
Clinical comparison of AAT Bioquest&rsquo;s PCT immunoassays and VIDAS<sup>&reg;</sup> B.R.A.H.M.S PCT assay: 70 clinical blood samples were separately tested using four MAb pairs on AAT Bioquest&rsquo;s CLIA platform and compared to a diagnostic kit from bioMerieux. Data from this study were analyzed using the Passing-Bablok regression method and are summarized in the following scatter plot. Results reveal good agreement between AAT Bioquest&rsquo;s immunoassay and comparison assay. The Mab pairs used are (capture-detection): Mab pair A: Cat# V100115 (Clone 3) - Cat# V100115 (Clone 2); Mab pair B: Cat# V100115 (Clone 4) - Cat# V100115 (Clone 2); Mab pair C: Cat# V100115 (Clone 3) - Cat# V100115 (Clone 5); and Mab pair D: Cat# V100115 (Clone 3) - Cat# V100115 (Clone 6).
Clinical comparison of AAT Bioquest&rsquo;s PCT immunoassays and VIDAS<sup>&reg;</sup> B.R.A.H.M.S PCT assay: 70 clinical blood samples were separately tested using four MAb pairs on AAT Bioquest&rsquo;s CLIA platform and compared to a diagnostic kit from bioMerieux. Data from this study were analyzed using the Passing-Bablok regression method and are summarized in the following scatter plot. Results reveal good agreement between AAT Bioquest&rsquo;s immunoassay and comparison assay. The Mab pairs used are (capture-detection): Mab pair A: Cat# V100115 (Clone 3) - Cat# V100115 (Clone 2); Mab pair B: Cat# V100115 (Clone 4) - Cat# V100115 (Clone 2); Mab pair C: Cat# V100115 (Clone 3) - Cat# V100115 (Clone 5); and Mab pair D: Cat# V100115 (Clone 3) - Cat# V100115 (Clone 6).
Clinical comparison of AAT Bioquest&rsquo;s PCT immunoassays and VIDAS<sup>&reg;</sup> B.R.A.H.M.S PCT assay: 70 clinical blood samples were separately tested using four MAb pairs on AAT Bioquest&rsquo;s CLIA platform and compared to a diagnostic kit from bioMerieux. Data from this study were analyzed using the Passing-Bablok regression method and are summarized in the following scatter plot. Results reveal good agreement between AAT Bioquest&rsquo;s immunoassay and comparison assay. The Mab pairs used are (capture-detection): Mab pair A: Cat# V100115 (Clone 3) - Cat# V100115 (Clone 2); Mab pair B: Cat# V100115 (Clone 4) - Cat# V100115 (Clone 2); Mab pair C: Cat# V100115 (Clone 3) - Cat# V100115 (Clone 5); and Mab pair D: Cat# V100115 (Clone 3) - Cat# V100115 (Clone 6).
Calibration curve for procalcitonin in sandwich chemiluminescence immunoassay (Six calibrator concentration: 0, 0.1, 0.5, 2, 10, 50 ng/mL). All monoclonal antibodies were tested in pairs as capture and detection antibodies to determine the best two-site MAb pairs for the development of quantitative sandwich immunoassays. Detection antibodies were labeled with horseradish peroxidase (HRP). The best selected antibody pairs for quantification of human procalcitonin are (capture-detection): Mab pair A: Cat# V100115 (Clone 3) - Cat# V100115 (Clone 2); Mab pair B: Cat# V100115 (Clone 4) - Cat# V100115 (Clone 2); Mab pair C: Cat# V100115 (Clone 3) - Cat# V100115 (Clone 5); and Mab pair D: Cat# V100115 (Clone 3) - Cat# V100115 (Clone 6).