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Anti-Platelet-activating factor acetylhydrolase (Lp-PLA2) antibody *Mouse anti-human, monoclonal IgG1*

Lipoprotein-associated phospholipase A2 (Lp-PLA2), or platelet-activating factor acetylhydrolase (PAF-AH), is a 45 kDa phospholipase enzyme produced by inflammatory cells such as mature macrophages and activated platelets. Lp-PLA2 is typically found present in the blood coupled to lipoproteins: 80% to LDL and 20% to HDL. It is essential for the hydrolysis of oxidized phospholipids, yielding pro-inflammatory lysophospholipids and oxidized fatty acids. In clinically biology, Lp-PLA2 is a significant biomarker associated with the development of atherosclerosis and cardiac disease. Anti-Lp-PLA2 monoclonal antibodies are highly specific for the detection of human Lp-PLA2 immunogens. They can be used for a broad range of immunoassays such as chemiluminescent immunoassay (CLIA), latex enhanced turbidimetric immunoassay (LETIA), ELISA and rapid sandwich immunoassays.

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of Anti-Platelet-activating factor acetylhydrolase (Lp-PLA2) antibody *Mouse anti-human, monoclonal IgG1* with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on October 31, 2024

Ordering information

Price
Unit size
50 ug
1 mg
Catalog Number
Quantity
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Antibody properties

Other names
Hostmouse
Reactivityhuman
ApplicationLETIA; ELISA; CLIA; LFIA

Storage, safety and handling

Intended useResearch Use Only (RUO)
Thermal stability profiles of four monoclonal antibodies: anti-human Lp-PLA2 monoclonal antibodies presented in PBS buffer (without any preservative) were stored at -20°C, 2-8°C and 37°C for 15 days.
Thermal stability profiles of four monoclonal antibodies: anti-human Lp-PLA2 monoclonal antibodies presented in PBS buffer (without any preservative) were stored at -20°C, 2-8°C and 37°C for 15 days.
Thermal stability profiles of four monoclonal antibodies: anti-human Lp-PLA2 monoclonal antibodies presented in PBS buffer (without any preservative) were stored at -20°C, 2-8°C and 37°C for 15 days.
<p>Calibration curve for Lp-PLA2 sandwich chemiluminescence immunoassay (CLIA): all MAbs were tested in pairs as capture and detection antibodies to select the best two-site MAb combinations for the development of a quantitative sandwich immunoassay. Detection antibodies were labeled with horseradish peroxidase (HRP). The best selected MAb combinations for the development of quantitative human Lp-PLA2 immunoassays are (capture-detection): MAb combination A:&nbsp; Cat# V100080 (Clone 1) - Cat# V100080 (Clone 3); MAb combination C:&nbsp; Cat# V100080 (Clone 3) - Cat# V100080 (Clone 1); MAb combination E:&nbsp; Cat# V100080 (Clone 4) - Cat# V100080 (Clone 5).</p> <p>&nbsp;</p> <p>MAb combination A:&nbsp; Cat# V100090 (Clone 1) - Cat# V100090 (Clone 3); MAb combination C:&nbsp; Cat# V100090 (Clone 3) - Cat# V100090 (Clone 1); MAb combination E:&nbsp; Cat# V100090 (Clone 4) - Cat# V100090 (Clone 5).</p> <p>&nbsp;</p> <p>MAb combination A:&nbsp; Cat# V100085 (Clone 1) - Cat# V100085 (Clone 3); MAb combination C:&nbsp; Cat# V100085 (Clone 3) - Cat# V100085 (Clone 1); MAb combination E:&nbsp; Cat# V100085 (Clone 4) - Cat# V100085 (Clone 5).</p>
Determination of clinical&nbsp;samples on CLIA using MAb combinations: 81 clinical&nbsp;samples from healthy donors and patients with atherosclerosis or cerebral infarction were detected using various Mab combinations in a CLIA Lp-PLA2 assay and a competitor&rsquo;s assay. The Mab combinations used are (capture-detection): MAb combination A:&nbsp; Cat# V100080 (Clone 1) - Cat# V100080 (Clone 3); MAb combination C:&nbsp; Cat# V100080 (Clone 3) - Cat# V100080 (Clone 1); MAb combination E:&nbsp; Cat# V100080 (Clone 4) - Cat# V100080 (Clone 5).

Documents

pdfSafety data sheet
pdfProduct protocol
pdfCertificate of analysis
Antibodies and Proteomics