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AAT Bioquest

Anti-C-reactive protein antibody *Mouse anti-human, monoclonal IgG2b*

C-reactive protein (CRP) is an annular, pentameric protein synthesized by the liver. It is present in blood plasma and is used primarily as a biomarker of inflammation. In response to inflammation, infection, or tissue damage, the level of CRP in human serum can increase 1000-fold. CRP also exhibits Ca2+ dependent binding to ligands, promote phagocytosis, and can induce production of hydrogen peroxide and inflammatory cytokines by monocytes. In clinical biology, CRP concentration can be used to evaluate the onset of cardiovascular disease. Anti-CRP monoclonal antibodies are highly specific for the detection of human C-reactive proteins. They can be used for a broad range of immunoassays such as lateral-flow immunoassay (LFIA), chemiluminescent immunoassay (CLIA), and latex enhanced turbidimetric immunoassays (LETIA).

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of Anti-C-reactive protein antibody *Mouse anti-human, monoclonal IgG2b* with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on October 31, 2024

Ordering information

Price
Unit size
50 ug
1 mg
Catalog Number
Quantity
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Additional ordering information

Telephone1-800-990-8053
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Emailsales@aatbio.com
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Antibody properties

Other names
Hostmouse
Reactivityhuman
ApplicationCLIA; LFIA

Storage, safety and handling

Intended useResearch Use Only (RUO)
Determination of clinical samples using AAT Bioquest’s mAb on CLIA platform: a study was performed where 48 specimens were tested using two antibody combinations and compared to a high-quality comparison assay.
Determination of clinical samples using AAT Bioquest’s mAb on CLIA platform: a study was performed where 48 specimens were tested using two antibody combinations and compared to a high-quality comparison assay.
Determination of clinical samples using AAT Bioquest’s mAb on CLIA platform: a study was performed where 48 specimens were tested using two antibody combinations and compared to a high-quality comparison assay.
Calibration curve for CRP in latex-enhanced turbidimetric immunoassay (LETIA): anti-CRP monoclonal antibodies (Cat#V100150) pre-coated onto latex beads were reacted with CRP proteins to form insoluble complexes, resulting in turbidity increasing. Absorbance increase was monitored by automatic biochemical analyzer.
Semi-quantitative detection of CRP protein in colloidal gold immunochromatogragphic assay: a set of CRP calibrators with concentrations of 0, 0.1, 0.5, 2, 10, 20, 50 and 100 mg/L were detected using an LFIA platform. The capture antibody was stripped on the nitrocellulose membrane, and the detection antibody was conjugated to colloidal gold. The best selected mAb combination is (capture-detection): Cat# V100150 (Clone 6) – Cat# V100150 (Clone 5).
Calibration curve for CRP in sandwich chemiluminescence immunoassay (CLIA): two monoclonal antibodies were tested in pairs as capture and detection antibodies for the development of a quantitative sandwich immunoassay. The mAb combination for quantification of human CRP is (capture-detection): Cat# V100150 (Clone 3) – Cat# V100150 (Clone 2).
Clinical comparison of AAT Bioquest’s CRP immunoassay and commercial diagnostic assay.