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AAT Bioquest

Anti-Beta-2-microglobulin (B2M) antibody *Mouse anti-human, monoclonal IgG1*

Human beta-2-microglobulin (B2M) is an 11.6 kDa non-glycosylated protein present in a variety of bodily fluids. It is a component of Class I MHC molecules and is necessary for the proper folding, binding of antigenic peptide, and surface expression of MHC-1 molecules. In long-term hemodialysis patients, B2M can accumulate to cause serious dialysis-related amyloidosis. Additionally, elevated levels of B2M are associated with rheumatoid arthritis, viral infections, cancer and aging. Anti-B2M monoclonal antibodies are highly specific for the detection of human B2M proteins. They can be used in a broad range of immunoassays such as latex enhanced turbidimetric immunoassays (LETIA), lateral-flow immunoassays (LFIA) and ELISA.

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of Anti-Beta-2-microglobulin (B2M) antibody *Mouse anti-human, monoclonal IgG1* with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on October 31, 2024

Ordering information

Price
Unit size
50 ug
1 mg
Catalog Number
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
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Antibody properties

Other names
Hostmouse
Reactivityhuman
ApplicationELISA; LFIIA

Physical properties

Solvent

Water

Storage, safety and handling

Intended useResearch Use Only (RUO)

Storage

Freeze (< -15 °C)
Linear calibration curve for B2M in latex-enhanced turbidimetric immunoassay (LETIA): human B2M was reacted with the anti-human B2M antibody-coated latex, resulting in agglutination and an increase in turbidity. Turbidity changes were monitored using a spectrometer to quantitatively measure the B2M concentration in the sample.
Linear calibration curve for B2M in latex-enhanced turbidimetric immunoassay (LETIA): human B2M was reacted with the anti-human B2M antibody-coated latex, resulting in agglutination and an increase in turbidity. Turbidity changes were monitored using a spectrometer to quantitatively measure the B2M concentration in the sample.
Linear calibration curve for B2M in latex-enhanced turbidimetric immunoassay (LETIA): human B2M was reacted with the anti-human B2M antibody-coated latex, resulting in agglutination and an increase in turbidity. Turbidity changes were monitored using a spectrometer to quantitatively measure the B2M concentration in the sample.
Thermal stability of anti-B2M monoclonal antibodies: anti-B2M monoclonal antibodies were stored at 4&deg;C and 37&deg;C for 14 days.
Clinical comparison between AAT Bioquest&rsquo;s B2M reagent and commercial kit.
<p>Clinical comparison of AAT Bioquest&rsquo;s B2M reagent and commercial LETIA kit: a B2M lateral-flow immunoassay (LFIA) using anti-B2M antibody (Cat# V100015; labeled with gold) and capture antibody was compared with a commercial B2M LETIA kit. Each assay was performed using 6 blood samples which were diluted by 50-fold.</p>
<p>&nbsp;(&ldquo;+&rdquo;: general positive, &ldquo;-&rdquo;: negative, LETIA: mg/L, &ldquo;C&rdquo;: control, &ldquo;T&rdquo;: test)</p>