Anti-Alpha-fetoprotein antibody *Mouse anti-human, monoclonal IgG1*
Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal development. It is widely used as diagnostic marker to screen for various developmental abnormalities and in adults, pancreatic cancer, lung cancer, liver cancer and liver cirrhosis. During pregnancy, high concentrations of AFP in the amniotic fluid may indicate severe congenital defects of a fetus, teratoma and fetal death. Anti-AFP monoclonal antibodies are highly specific for the detection of human AFP immunogens. They can be used for a broad range of immunoassays chemiluminescence immunoassays (CLIA) and rapid sandwich immunoassays.
![<p>Graphs showing the determination of clinical samples using two antibody combinations on CLIA platform and compared to a high-quality comparison assay. Results indicate AAT Bioquest’s mAb combinations A and B can be applied to double-mAb-sandwich-immunoassays.</p>
<p>mAb combination A: Clone â‘¢ - V100065</p>
<p>mAb combination B: Clone â‘¡ - V100065</p>](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fanti-alpha-fetoprotein-antibody-mouse-anti-human-monoclonal-igg1%2Ffigure-for-anti-alpha-fetoprotein-antibody-mouse-anti-human-monoclonal-igg1_i4Fhm.jpg&w=640&q=75)
![<p>Graphs showing the determination of clinical samples using two antibody combinations on CLIA platform and compared to a high-quality comparison assay. Results indicate AAT Bioquest’s mAb combinations A and B can be applied to double-mAb-sandwich-immunoassays.</p>
<p>mAb combination A: Clone â‘¢ - V100065</p>
<p>mAb combination B: Clone â‘¡ - V100065</p>](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fanti-alpha-fetoprotein-antibody-mouse-anti-human-monoclonal-igg1%2Ffigure-for-anti-alpha-fetoprotein-antibody-mouse-anti-human-monoclonal-igg1_i4Fhm.jpg&w=640&q=75)
![<p>Graphs showing the determination of clinical samples using two antibody combinations on CLIA platform and compared to a high-quality comparison assay. Results indicate AAT Bioquest’s mAb combinations A and B can be applied to double-mAb-sandwich-immunoassays.</p>
<p>mAb combination A: Clone â‘¢ - V100065</p>
<p>mAb combination B: Clone â‘¡ - V100065</p>](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fanti-alpha-fetoprotein-antibody-mouse-anti-human-monoclonal-igg1%2Ffigure-for-anti-alpha-fetoprotein-antibody-mouse-anti-human-monoclonal-igg1_i4Fhm.jpg&w=128&q=25)
![<p>Calibration curves for AFP in a sandwich chemiluminescence immunoassay (CLIA). Monoclonal antibodies were tested in pairs as capture and detection antibodies to determine the best two-site mAb combinations for the development of a quantitative sandwich immunoassay. The best selected mAb combinations for quantification of human AFP are (capture-detection respectively):</p>
<p>mAb combination A: Clone â‘¢ - V100065</p>
<p>mAb combination B: Clone â‘¡ - V100065</p>](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fanti-alpha-fetoprotein-antibody-mouse-anti-human-monoclonal-igg1%2Ffigure-for-anti-alpha-fetoprotein-antibody-mouse-anti-human-monoclonal-igg1_PRE4S.jpg&w=128&q=25)
Conjugation
We provide custom conjugation services for this antibody (eg. labeling of Anti-Alpha-fetoprotein antibody *Mouse anti-human, monoclonal IgG1* with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.