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Ac-LKKR-AMC
Ordering information
| Price | |
| Catalog Number | |
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1084.99 |
| Solvent | DMSO |
Spectral properties
| Excitation (nm) | 341 |
| Emission (nm) | 441 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| Overview |  SDSProtocol |
See also: Proteases
Molecular weight 1084.99 | Excitation (nm) 341 | Emission (nm) 441 |
Ac-Leu-Lys-Lys-Arg-AMC is a fluorogenic aminopeptidase and endopeptidase substrate. It might be used as a fluorogenic probe for detecting thrombin and metacaspases. Metacaspases are found in plants, fungi, and protozoa. They are cysteine-proteases and are distantly related to metazoan caspases. The protease activity is quantified by release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380 nm and emits at 440-460 nm.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Ac-LKKR-AMC to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 92.167 µL | 460.834 µL | 921.667 µL | 4.608 mL | 9.217 mL |
| 5 mM | 18.433 µL | 92.167 µL | 184.333 µL | 921.667 µL | 1.843 mL |
| 10 mM | 9.217 µL | 46.083 µL | 92.167 µL | 460.834 µL | 921.667 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Product Family
| Name | Excitation (nm) | Emission (nm) |
| Ac-DEVD-AMC *CAS 169332-61-0* | 341 | 441 |
| Ac-IETD-AMC | 341 | 441 |
| Ac-LEHD-AMC *CAS 292633-16-0* | 341 | 441 |
| Ac-Leu-AMC | 341 | 441 |
Images
Figure 1. Ac-Leu-Lys-Lys-Arg-AMC is a fluorogenic aminopeptidase and endopeptidase substrate. The protease activity is quantified by release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380 nm and emits at 440-460 nm.
References
View all 50 references: Citation Explorer
Refolding of metacaspase 5 from Trypanosoma cruzi, structural characterization and the influence of c-terminal in protein recombinant production.
Authors: De Lima, Jhenifer Yonara and Santos, Marlon Dias Mariano Dos and Andreassa, Emanuella de Castro and Kurt, Louise Ulrich and Carvalho, Paulo Costa and De Souza, Tatiana de Arruda Campos Brasil
Journal: Protein expression and purification (2022): 106007
Authors: De Lima, Jhenifer Yonara and Santos, Marlon Dias Mariano Dos and Andreassa, Emanuella de Castro and Kurt, Louise Ulrich and Carvalho, Paulo Costa and De Souza, Tatiana de Arruda Campos Brasil
Journal: Protein expression and purification (2022): 106007
Detection of Damage-Activated Metacaspase Activity by Western Blot in Plants.
Authors: Stael, Simon and Miller, Luke P and Fernández-Fernández, Álvaro D and Van Breusegem, Frank
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 127-137
Authors: Stael, Simon and Miller, Luke P and Fernández-Fernández, Álvaro D and Van Breusegem, Frank
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 127-137
Expression and Purification of the Type II Metacaspase from a Unicellular Green Alga Chlamydomonas reinhardtii.
Authors: Sabljić, Igor and Zou, Yong and Klemenčič, Marina and Funk, Christiane and Ståhlberg, Jerry and Bozhkov, Peter
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 13-20
Authors: Sabljić, Igor and Zou, Yong and Klemenčič, Marina and Funk, Christiane and Ståhlberg, Jerry and Bozhkov, Peter
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 13-20
Expression and Purification of the Type I Metacaspase from a Cryptophyte Guillardia theta , GtMCA-I.
Authors: Štrancar, Vida and van Midden, Katarina Petra and Klemenčič, Marina and Funk, Christiane
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 1-11
Authors: Štrancar, Vida and van Midden, Katarina Petra and Klemenčič, Marina and Funk, Christiane
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 1-11
Dissecting The role of Plasmodium metacaspase-2 in malaria gametogenesis and sporogony.
Authors: Kumari, Vandana and Prasad, Kona Madhavinadha and Kalia, Inderjeet and Sindhu, Gagandeep and Dixit, Rajnikant and Rawat, Diwan S and Singh, O P and Singh, Agam P and Pandey, Kailash C
Journal: Emerging microbes & infections (2022): 938-955
Authors: Kumari, Vandana and Prasad, Kona Madhavinadha and Kalia, Inderjeet and Sindhu, Gagandeep and Dixit, Rajnikant and Rawat, Diwan S and Singh, O P and Singh, Agam P and Pandey, Kailash C
Journal: Emerging microbes & infections (2022): 938-955
Plasmodium falciparum metacaspase-2 capture its natural substrate in a non-canonical way.
Authors: Vandana, and Pandey, Rajan and Srinivasan, E and Kalia, Inderjeet and Singh, Agam P and Saxena, Ajay and Rajaekaran, R and Gupta, Dinesh and Pandey, Kailash C
Journal: Journal of biochemistry (2021): 639-653
Authors: Vandana, and Pandey, Rajan and Srinivasan, E and Kalia, Inderjeet and Singh, Agam P and Saxena, Ajay and Rajaekaran, R and Gupta, Dinesh and Pandey, Kailash C
Journal: Journal of biochemistry (2021): 639-653
Identification of a Metacaspase Gene in the Bloom-Forming Dinoflagellate Prorocentrum minimum and its Putative Function Involved in Programmed Cell Death.
Authors: Wang, Hui and Ki, Jang-Seu
Journal: Current microbiology (2021): 3577-3585
Authors: Wang, Hui and Ki, Jang-Seu
Journal: Current microbiology (2021): 3577-3585
Screening and Identification of Metacaspase Inhibitors: Evaluation of Inhibition Mechanism and Trypanocidal Activity.
Authors: Pérez, Brian and Bouvier, León A and Cazzulo, Juan José and Agüero, Fernán and Salas-Sarduy, Emir and Alvarez, Vanina E
Journal: Antimicrobial agents and chemotherapy (2021)
Authors: Pérez, Brian and Bouvier, León A and Cazzulo, Juan José and Agüero, Fernán and Salas-Sarduy, Emir and Alvarez, Vanina E
Journal: Antimicrobial agents and chemotherapy (2021)
Biochemical Characterization of a Novel Redox-Regulated Metacaspase in a Marine Diatom.
Authors: Graff van Creveld, Shiri and Ben-Dor, Shifra and Mizrachi, Avia and Alcolombri, Uria and Hopes, Amanda and Mock, Thomas and Rosenwasser, Shilo and Vardi, Assaf
Journal: Frontiers in microbiology (2021): 688199
Authors: Graff van Creveld, Shiri and Ben-Dor, Shifra and Mizrachi, Avia and Alcolombri, Uria and Hopes, Amanda and Mock, Thomas and Rosenwasser, Shilo and Vardi, Assaf
Journal: Frontiers in microbiology (2021): 688199
Suppression of Metacaspase- and Autophagy-Dependent Cell Death Improves Stress-Induced Microspore Embryogenesis in Brassica napus.
Authors: Berenguer, Eduardo and Minina, Elena A and Carneros, Elena and B R Ny, Ivett and Bozhkov, Peter V and Testillano, Pilar S
Journal: Plant & cell physiology (2021): 2097-2110
Authors: Berenguer, Eduardo and Minina, Elena A and Carneros, Elena and B R Ny, Ivett and Bozhkov, Peter V and Testillano, Pilar S
Journal: Plant & cell physiology (2021): 2097-2110
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
An Increase in Plasma Homovanillic Acid with Cocoa Extract Consumption Is Associated with the Alleviation of Depressive Symptoms in Overweight or Obese Adults
Attenuation of lysyl oxidase and collagen gene expression in keratoconus patient corneal epithelium corresponds to disease severity
β-adrenoceptors are upregulated in human melanoma and their activation releases pro-tumorigenic cytokines and metalloproteases in melanoma cell lines
Abbreviation of Common Chemical Compounds Related to Peptides
An Increase in Plasma Homovanillic Acid with Cocoa Extract Consumption Is Associated with the Alleviation of Depressive Symptoms in Overweight or Obese Adults
Attenuation of lysyl oxidase and collagen gene expression in keratoconus patient corneal epithelium corresponds to disease severity
β-adrenoceptors are upregulated in human melanoma and their activation releases pro-tumorigenic cytokines and metalloproteases in melanoma cell lines