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Ac-LKKR-AMC
Ac-Leu-Lys-Lys-Arg-AMC is a fluorogenic aminopeptidase and endopeptidase substrate. It might be used as a fluorogenic probe for detecting thrombin and metacaspases. Metacaspases are found in plants, fungi, and protozoa. They are cysteine-proteases and are distantly related to metazoan caspases. The protease activity is quantified by release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380 nm and emits at 440-460 nm.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) |
| Ac-DEVD-AMC *CAS 169332-61-0* | 341 | 441 |
| Ac-IETD-AMC | 341 | 441 |
| Ac-LEHD-AMC *CAS 292633-16-0* | 341 | 441 |
| Ac-Leu-AMC | 341 | 441 |
References
View all 50 references: Citation Explorer
Refolding of metacaspase 5 from Trypanosoma cruzi, structural characterization and the influence of c-terminal in protein recombinant production.
Authors: De Lima, Jhenifer Yonara and Santos, Marlon Dias Mariano Dos and Andreassa, Emanuella de Castro and Kurt, Louise Ulrich and Carvalho, Paulo Costa and De Souza, Tatiana de Arruda Campos Brasil
Journal: Protein expression and purification (2022): 106007
Authors: De Lima, Jhenifer Yonara and Santos, Marlon Dias Mariano Dos and Andreassa, Emanuella de Castro and Kurt, Louise Ulrich and Carvalho, Paulo Costa and De Souza, Tatiana de Arruda Campos Brasil
Journal: Protein expression and purification (2022): 106007
Detection of Damage-Activated Metacaspase Activity by Western Blot in Plants.
Authors: Stael, Simon and Miller, Luke P and Fernández-Fernández, Álvaro D and Van Breusegem, Frank
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 127-137
Authors: Stael, Simon and Miller, Luke P and Fernández-Fernández, Álvaro D and Van Breusegem, Frank
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 127-137
Expression and Purification of the Type II Metacaspase from a Unicellular Green Alga Chlamydomonas reinhardtii.
Authors: Sabljić, Igor and Zou, Yong and Klemenčič, Marina and Funk, Christiane and Ståhlberg, Jerry and Bozhkov, Peter
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 13-20
Authors: Sabljić, Igor and Zou, Yong and Klemenčič, Marina and Funk, Christiane and Ståhlberg, Jerry and Bozhkov, Peter
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 13-20
Expression and Purification of the Type I Metacaspase from a Cryptophyte Guillardia theta , GtMCA-I.
Authors: Štrancar, Vida and van Midden, Katarina Petra and Klemenčič, Marina and Funk, Christiane
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 1-11
Authors: Štrancar, Vida and van Midden, Katarina Petra and Klemenčič, Marina and Funk, Christiane
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 1-11
Dissecting The role of Plasmodium metacaspase-2 in malaria gametogenesis and sporogony.
Authors: Kumari, Vandana and Prasad, Kona Madhavinadha and Kalia, Inderjeet and Sindhu, Gagandeep and Dixit, Rajnikant and Rawat, Diwan S and Singh, O P and Singh, Agam P and Pandey, Kailash C
Journal: Emerging microbes & infections (2022): 938-955
Authors: Kumari, Vandana and Prasad, Kona Madhavinadha and Kalia, Inderjeet and Sindhu, Gagandeep and Dixit, Rajnikant and Rawat, Diwan S and Singh, O P and Singh, Agam P and Pandey, Kailash C
Journal: Emerging microbes & infections (2022): 938-955
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