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Ac-LKKR-AMC

Ac-Leu-Lys-Lys-Arg-AMC is a fluorogenic aminopeptidase and endopeptidase substrate. It might be used as a fluorogenic probe for detecting thrombin and metacaspases. Metacaspases are found in plants, fungi, and protozoa. They are cysteine-proteases and are distantly related to metazoan caspases. The protease activity is quantified by release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380 nm and emits at 440-460 nm.

Spectrum

Product family

NameExcitation (nm)Emission (nm)
Ac-DEVD-AMC *CAS 169332-61-0*341441
Ac-IETD-AMC341441
Ac-LEHD-AMC *CAS 292633-16-0*341441
Ac-Leu-AMC341441

References

View all 50 references: Citation Explorer
Refolding of metacaspase 5 from Trypanosoma cruzi, structural characterization and the influence of c-terminal in protein recombinant production.
Authors: De Lima, Jhenifer Yonara and Santos, Marlon Dias Mariano Dos and Andreassa, Emanuella de Castro and Kurt, Louise Ulrich and Carvalho, Paulo Costa and De Souza, Tatiana de Arruda Campos Brasil
Journal: Protein expression and purification (2022): 106007
Detection of Damage-Activated Metacaspase Activity by Western Blot in Plants.
Authors: Stael, Simon and Miller, Luke P and Fernández-Fernández, Álvaro D and Van Breusegem, Frank
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 127-137
Expression and Purification of the Type II Metacaspase from a Unicellular Green Alga Chlamydomonas reinhardtii.
Authors: Sabljić, Igor and Zou, Yong and Klemenčič, Marina and Funk, Christiane and Ståhlberg, Jerry and Bozhkov, Peter
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 13-20
Expression and Purification of the Type I Metacaspase from a Cryptophyte Guillardia theta , GtMCA-I.
Authors: Štrancar, Vida and van Midden, Katarina Petra and Klemenčič, Marina and Funk, Christiane
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 1-11
Dissecting The role of Plasmodium metacaspase-2 in malaria gametogenesis and sporogony.
Authors: Kumari, Vandana and Prasad, Kona Madhavinadha and Kalia, Inderjeet and Sindhu, Gagandeep and Dixit, Rajnikant and Rawat, Diwan S and Singh, O P and Singh, Agam P and Pandey, Kailash C
Journal: Emerging microbes & infections (2022): 938-955
Page updated on December 17, 2024

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Catalog Number13446
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Physical properties

Molecular weight

1084.99

Solvent

DMSO

Spectral properties

Excitation (nm)

341

Emission (nm)

441

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
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