Ac-LKKR-AMC

Ac-Leu-Lys-Lys-Arg-AMC is a fluorogenic aminopeptidase and endopeptidase substrate. It might be used as a fluorogenic probe for detecting thrombin and metacaspases. Metacaspases are found in plants, fungi, and protozoa. They are cysteine-proteases and are distantly related to metazoan caspases. The protease activity is quantified by release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380 nm and emits at 440-460 nm.

Ac-Leu-Lys-Lys-Arg-AMC is a fluorogenic aminopeptidase and endopeptidase substrate. The protease activity is quantified by release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380 nm and emits at 440-460 nm.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13446	1 mg	Price

References

View all 50 references: Citation Explorer

Refolding of metacaspase 5 from Trypanosoma cruzi, structural characterization and the influence of c-terminal in protein recombinant production.

Authors:

De Lima, Jhenifer Yonara and Santos, Marlon Dias Mariano Dos and Andreassa, Emanuella de Castro and Kurt, Louise Ulrich and Carvalho, Paulo Costa and De Souza, Tatiana de Arruda Campos Brasil

Journal:

Protein expression and purification (2022): 106007

Detection of Damage-Activated Metacaspase Activity by Western Blot in Plants.

Authors:

Stael, Simon and Miller, Luke P and Fernández-Fernández, Álvaro D and Van Breusegem, Frank

Journal:

Methods in molecular biology (Clifton, N.J.) (2022): 127-137

Expression and Purification of the Type II Metacaspase from a Unicellular Green Alga Chlamydomonas reinhardtii.

Authors:

Sabljić, Igor and Zou, Yong and Klemenčič, Marina and Funk, Christiane and Ståhlberg, Jerry and Bozhkov, Peter

Journal:

Methods in molecular biology (Clifton, N.J.) (2022): 13-20

Expression and Purification of the Type I Metacaspase from a Cryptophyte Guillardia theta , GtMCA-I.

Authors:

Štrancar, Vida and van Midden, Katarina Petra and Klemenčič, Marina and Funk, Christiane

Journal:

Methods in molecular biology (Clifton, N.J.) (2022): 1-11

Dissecting The role of Plasmodium metacaspase-2 in malaria gametogenesis and sporogony.

Authors:

Kumari, Vandana and Prasad, Kona Madhavinadha and Kalia, Inderjeet and Sindhu, Gagandeep and Dixit, Rajnikant and Rawat, Diwan S and Singh, O P and Singh, Agam P and Pandey, Kailash C

Journal:

Emerging microbes & infections (2022): 938-955

Physical properties

Molecular weight	1084.99
Solvent	DMSO

Spectral properties

Excitation (nm)	341
Emission (nm)	441

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 25, 2025