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Ac-Leu-AMC
Ac-Leu-AMC is a fluorogenic substrate for leucine aminopeptidase. Upon the hydrolysis by proteases, Leu-AMC releases the strongly fluorescent AMC fluorophore that can be readily detected with Ex/Em = 350/430 nm. Leucine aminopeptidase is an extracellular enzyme found abundantly in natural aquatic systems. It is one of the various enzymes used by bacteria for protein hydrolysis. Ac-L-Leucine-7-amido-4-methylcoumarin might be used as a substrate in fluorescence-based soil assays to determine leucine aminopeptidase activity of both Plasmodium falciparum M1 (PfA-M1) and PfA-M17 enzymes.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) |
| Ac-DEVD-AMC *CAS 169332-61-0* | 341 | 441 |
| Ac-IETD-AMC | 341 | 441 |
| Ac-LEHD-AMC *CAS 292633-16-0* | 341 | 441 |
| Ac-LKKR-AMC | 341 | 441 |
References
View all 9 references: Citation Explorer
Skeletocutins M-Q: biologically active compounds from the fruiting bodies of the basidiomycete Skeletocutis sp. collected in Africa.
Authors: Cheng, Tian and Chepkirui, Clara and Decock, Cony and Matasyoh, Josphat C and Stadler, Marc
Journal: Beilstein journal of organic chemistry (2019): 2782-2789
Authors: Cheng, Tian and Chepkirui, Clara and Decock, Cony and Matasyoh, Josphat C and Stadler, Marc
Journal: Beilstein journal of organic chemistry (2019): 2782-2789
The Biochemical and Functional Characterization of M28 Aminopeptidase Protein Secreted by Acanthamoeba spp. on Host Cell Interaction.
Authors: Huang, Jian-Ming and Chang, Yao-Tsung and Lin, Wei-Chen
Journal: Molecules (Basel, Switzerland) (2019)
Authors: Huang, Jian-Ming and Chang, Yao-Tsung and Lin, Wei-Chen
Journal: Molecules (Basel, Switzerland) (2019)
Structural and functional highlights of methionine aminopeptidase 2 from Leishmania donovani.
Authors: Bhat, Saleem Yousuf and Dey, Arijit and Qureshi, Insaf A
Journal: International journal of biological macromolecules (2018): 940-954
Authors: Bhat, Saleem Yousuf and Dey, Arijit and Qureshi, Insaf A
Journal: International journal of biological macromolecules (2018): 940-954
Discovery of a pyruvylated peptide-metabolizing enzyme using a fluorescent substrate-based protein discovery technique.
Authors: Yoshioka, Kentaro and Komatsu, Toru and Hanaoka, Kenjiro and Ueno, Tasuku and Terai, Takuya and Nagano, Tetsuo and Urano, Yasuteru
Journal: Chemical communications (Cambridge, England) (2016): 4377-80
Authors: Yoshioka, Kentaro and Komatsu, Toru and Hanaoka, Kenjiro and Ueno, Tasuku and Terai, Takuya and Nagano, Tetsuo and Urano, Yasuteru
Journal: Chemical communications (Cambridge, England) (2016): 4377-80
A quantitative technique for determining proteases and their substrate specificities and pH optima in crude enzyme extracts.
Authors: Budic, Maruska and Kidric, Marjetka and Meglic, Vladimir and Cigić, Blaz
Journal: Analytical biochemistry (2009): 56-62
Authors: Budic, Maruska and Kidric, Marjetka and Meglic, Vladimir and Cigić, Blaz
Journal: Analytical biochemistry (2009): 56-62
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