6-JOE acid

6-JOE has similar absorption and emission spectra to those of rhodamine 6G, and its SE is predominantly used in automated DNA sequencing. It is also used to label nucleotides. 6-JOE acid is the most economic form to make a conjugate with amino-containing biomolecules.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
6-JOE, SE [6-Carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein, succinimidyl ester] CAS#: 113394-23-3	520	545	75000¹	-
6-OG488 acid [equivalent to Oregon Green® 488 carboxylic acid, 6-isomer]	498	526	76000	0.12
6-JOE Phosphoramidite	520	545	75000¹	-
6-ROXtra™ acid	578	595	-	-
6-HEX acid	533	559	73000	0.15
6-TET Acid	521	542	73000	0.13

References

View all 4 references: Citation Explorer

Optical Biosensing System for the Detection of Survivin mRNA in Colorectal Cancer Cells Using a Graphene Oxide Carrier-Bound Oligonucleotide Molecular Beacon.
Authors: Ratajczak, Katarzyna and Krazinski, Bartlomiej E and Kowalczyk, Anna E and Dworakowska, Beata and Jakiela, Slawomir and Stobiecka, Magdalena
Journal: Nanomaterials (Basel, Switzerland) (2018)

Hairpin-Hairpin Molecular Beacon Interactions for Detection of Survivin mRNA in Malignant SW480 Cells.
Authors: Ratajczak, Katarzyna and Krazinski, Bartlomiej E and Kowalczyk, Anna E and Dworakowska, Beata and Jakiela, Slawomir and Stobiecka, Magdalena
Journal: ACS applied materials & interfaces (2018): 17028-17039

Molecular beacons with JOE dye: Influence of linker and 3' couple quencher.
Authors: Tsybulsky, Dmitry A and Kvach, Maksim V and Ryazantsev, Dmitry Y and Aparin, Ilya O and Stakheev, Alexander A and Prokhorenko, Igor A and Martynenko, Yury V and Gontarev, Sergey V and Formanovsky, Andrey A and Zatsepin, Timofei S and Shmanai, Vadim V and Korshun, Vladimir A and Zavriev, Sergey K
Journal: Molecular and cellular probes (2016): 285-290

Short PNA molecular beacons for real-time PCR allelic discrimination of single nucleotide polymorphisms.
Authors: Petersen, Kenneth and Vogel, Ulla and Rockenbauer, Eszter and Nielsen, Kirsten Vang and Kølvraa, Steen and Bolund, Lars and Nexø, Bjørn
Journal: Molecular and cellular probes (2004): 117-22

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