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6-JOE acid

6-JOE has similar absorption and emission spectra to those of rhodamine 6G, and its SE is predominantly used in automated DNA sequencing. It is also used to label nucleotides. 6-JOE acid is the most economic form to make a conjugate with amino-containing biomolecules.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
6-JOE, SE [6-Carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein, succinimidyl ester] *CAS#: 113394-23-3*520545750001-
6-OG488 acid [equivalent to Oregon Green® 488 carboxylic acid, 6-isomer]498526760000.12
6-JOE Phosphoramidite520545750001-
6-ROXtra™ acid578595--
6-HEX acid533559730000.15
6-TET Acid521542730000.13

References

View all 4 references: Citation Explorer
Optical Biosensing System for the Detection of Survivin mRNA in Colorectal Cancer Cells Using a Graphene Oxide Carrier-Bound Oligonucleotide Molecular Beacon.
Authors: Ratajczak, Katarzyna and Krazinski, Bartlomiej E and Kowalczyk, Anna E and Dworakowska, Beata and Jakiela, Slawomir and Stobiecka, Magdalena
Journal: Nanomaterials (Basel, Switzerland) (2018)
Hairpin-Hairpin Molecular Beacon Interactions for Detection of Survivin mRNA in Malignant SW480 Cells.
Authors: Ratajczak, Katarzyna and Krazinski, Bartlomiej E and Kowalczyk, Anna E and Dworakowska, Beata and Jakiela, Slawomir and Stobiecka, Magdalena
Journal: ACS applied materials & interfaces (2018): 17028-17039
Molecular beacons with JOE dye: Influence of linker and 3' couple quencher.
Authors: Tsybulsky, Dmitry A and Kvach, Maksim V and Ryazantsev, Dmitry Y and Aparin, Ilya O and Stakheev, Alexander A and Prokhorenko, Igor A and Martynenko, Yury V and Gontarev, Sergey V and Formanovsky, Andrey A and Zatsepin, Timofei S and Shmanai, Vadim V and Korshun, Vladimir A and Zavriev, Sergey K
Journal: Molecular and cellular probes (2016): 285-290
Short PNA molecular beacons for real-time PCR allelic discrimination of single nucleotide polymorphisms.
Authors: Petersen, Kenneth and Vogel, Ulla and Rockenbauer, Eszter and Nielsen, Kirsten Vang and Kølvraa, Steen and Bolund, Lars and Nexø, Bjørn
Journal: Molecular and cellular probes (2004): 117-22
Page updated on October 30, 2024

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Physical properties

Molecular weight

505.26

Solvent

DMSO

Spectral properties

Extinction coefficient (cm -1 M -1)

750001

Excitation (nm)

520

Emission (nm)

545

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
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