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5-(and-6)-Carboxy SNARF-1 Maleimide
5-(and-6)-Carboxy SNARF-1 Maleimide is an excellent thiol-reactive building block that can be used for developing SNARF-1 pH probes. It can be readily conjugated to peptides, antibodies and other thiol-containing molecules such as thiol-modified oligos. SNARF-1 has pKa of ∼7.5, thus extremely useful for monitoring pH changes in the range of 6.5 and 8.5. It exhibits a significant pH-dependent emission shift from yellow orange to deep red fluorescence under acidic and basic conditions, respectively. The ratioable pH dependent fluorescence intensity change allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH in cells and other biological media.
References
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Quantification of bone marrow interstitial pH and calcium concentration by intravital ratiometric imaging.
Authors: Yeh, S-C A and Hou, J and Wu, J W and Yu, S and Zhang, Y and Belfield, K D and Camargo, F D and Lin, C P
Journal: Nature communications (2022): 393
Authors: Yeh, S-C A and Hou, J and Wu, J W and Yu, S and Zhang, Y and Belfield, K D and Camargo, F D and Lin, C P
Journal: Nature communications (2022): 393
Calibration and measurement of mitochondrial pH in intact adult rat cardiomyocytes.
Authors: Gao, Meng and Qin, Yuan and Li, Anqi and Liu, Hanyu and Chen, Lei and Liu, Bilin and Zhang, Ying and Gao, Yufei and Gong, Guohua
Journal: STAR protocols (2021): 100543
Authors: Gao, Meng and Qin, Yuan and Li, Anqi and Liu, Hanyu and Chen, Lei and Liu, Bilin and Zhang, Ying and Gao, Yufei and Gong, Guohua
Journal: STAR protocols (2021): 100543
Mitochondrial matrix pH acidifies during anoxia and is maintained by the F1Fo-ATPase in anoxia-tolerant painted turtle cortical neurons.
Authors: Hawrysh, Peter John and Buck, Leslie Thomas
Journal: FEBS open bio (2019): 571-581
Authors: Hawrysh, Peter John and Buck, Leslie Thomas
Journal: FEBS open bio (2019): 571-581
Energy transfer chemiluminescence for ratiometric pH imaging.
Authors: An, Weiwei and Mason, Ralph P and Lippert, Alexander R
Journal: Organic & biomolecular chemistry (2018): 4176-4182
Authors: An, Weiwei and Mason, Ralph P and Lippert, Alexander R
Journal: Organic & biomolecular chemistry (2018): 4176-4182
Improved pH-ratiometry for the three-dimensional mapping of pH microenvironments in biofilms under flow conditions.
Authors: Schlafer, Sebastian and Baelum, Vibeke and Dige, Irene
Journal: Journal of microbiological methods (2018): 194-200
Authors: Schlafer, Sebastian and Baelum, Vibeke and Dige, Irene
Journal: Journal of microbiological methods (2018): 194-200
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