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5-(and-6)-Carboxy SNARF-1 Alkyne
5-(and-6)-Carboxy SNARF-1 alkyne is an excellent azido-reactive building block that can be used for developing SNARF-1 pH probes via the well-known click chemistry. It can be readily conjugated to peptides, antibodies and other biomolecules that are modified with an azido group. SNARF-1 has pKa of ∼7.5, thus extremely useful for monitoring pH changes in the range of 6.5 and 8.5. It exhibits a significant pH-dependent emission shift from yellow orange to deep red fluorescence under acidic and basic conditions, respectively. The ratioable pH dependent fluorescence intensity change allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH in cells and other biological media.
References
View all 46 references: Citation Explorer
Studying Proton Gradients Across the Nuclear Envelope.
Authors: Martínez-Zaguilán, Raul and Sennoune, Souad R
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 47-63
Authors: Martínez-Zaguilán, Raul and Sennoune, Souad R
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 47-63
A Novel Nicotinamide Adenine Dinucleotide Correction Method for Intracellular Ca2+ Measurement with Fura-2-Analog in Live Cells.
Authors: Lee, Jeong Hoon and Ha, Jeong Mi and Ho, Quynh Mai and Leem, Chae Hun
Journal: Journal of visualized experiments : JoVE (2019)
Authors: Lee, Jeong Hoon and Ha, Jeong Mi and Ho, Quynh Mai and Leem, Chae Hun
Journal: Journal of visualized experiments : JoVE (2019)
Sensing Parts per Million Level Ammonia and Parts per Billion Level Acetic Acid in the Gas Phase by Common Black Film with a Fluorescent pH Probe.
Authors: Fu, Jingni and Zhang, Luning
Journal: Analytical chemistry (2018): 1356-1362
Authors: Fu, Jingni and Zhang, Luning
Journal: Analytical chemistry (2018): 1356-1362
Imaging of Mitochondrial pH Using SNARF-1.
Authors: Ramshesh, Venkat K and Lemasters, John J
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 351-356
Authors: Ramshesh, Venkat K and Lemasters, John J
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 351-356
Ratiometric Imaging of Extracellular pH in Dental Biofilms.
Authors: Schlafer, Sebastian and Dige, Irene
Journal: Journal of visualized experiments : JoVE (2016)
Authors: Schlafer, Sebastian and Dige, Irene
Journal: Journal of visualized experiments : JoVE (2016)
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