DBCO NHS Ester

Cu-free click reaction, i.e., the strain-promoted alkyne-azide cycloaddition (SPAAC) is a bioorthogonal reaction utilizing a pair of reagents, cyclooctynes and azides that exclusively and efficiently react with each other while remain inert to naturally occurring functional groups such as hydroxy, amino and thiol groups. SPAAC enables labeling a wide variety of biomolecules without any auxiliary reagents in an aqueous and otherwise complex chemical environment through the formation of a stable triazole. Among the large number of known cyclooctynes, dibenzocyclooctyne (DBCO) compounds comprise a class of reagents that possesses reasonably fast kinetics and good stability in aqueous buffers. Within physiological temperature and pH ranges, the DBCO group will not react with thiol, amino or hydroxy groups that are naturally present in many biomolecules. DBCO NHS Ester is one of the most common reagents used for introducing DBCO group into a biomolecule via the modification of the amino groups.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DBCO NHS Ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	248.503 µL	1.243 mL	2.485 mL	12.425 mL	24.85 mL
5 mM	49.701 µL	248.503 µL	497.006 µL	2.485 mL	4.97 mL
10 mM	24.85 µL	124.251 µL	248.503 µL	1.243 mL	2.485 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
XFD488 NHS Ester Same Structure to Alexa Fluor™ 488 NHS Ester	499	520	71000	0.30	0.11
XFD350 NHS Ester Same Structure to Alexa Fluor™ 350 NHS Ester	343	441	19000	0.25	0.19
XFD532 NHS Ester Same Structure to Alexa Fluor™ 532 NHS Ester	534	553	81000	0.24	0.09
XFD594 NHS Ester Same Structure to Alexa Fluor™ 594 NHS Ester	590	618	90000	0.43	0.56
QXY21 NHS ester [equivalent to QSY-21 NHS ester]	-	-	89000¹	-	0.32
XFD555 NHS Ester Same Structure to Alexa Fluor™ 555 NHS Ester	553	568	150000	0.08	0.08
XFD647 NHS Ester Same Structure to Alexa Fluor™ 647 NHS Ester	650	671	239000	0.00	0.03
XFD680 NHS Ester Same Structure to Alexa Fluor™ 680 NHS Ester	681	704	184000	0.00	0.05
XFD700 NHS Ester Same Structure to Alexa Fluor™ 700 NHS Ester	696	719	192000	0.00	0.07
XFD750 NHS Ester Same Structure to Alexa Fluor™ 750 NHS Ester	752	776	240000	0.00	0.04
Cy5DIGE NHS ester	651	670	250000¹	0.02	0.03
Cy2DIGE NHS ester	492	508	150000	0.08	0.15
Cy3DIGE NHS ester	555	569	150000¹	0.07	0.073
XFD546 NHS Ester Same Structure to Alexa Fluor™ 546 NHS Ester	561	572	112000	0.21	0.12
XFD568 NHS Ester Same Structure to Alexa Fluor™ 568 NHS Ester	579	603	91300	0.45	0.46
XFD514 NHS Ester Same Structure to Alexa Fluor™ 514 NHS Ester	518	543	80000	0.31	0.18
QXY7 NHS ester [equivalent to QSY-7 NHS ester]	-	-	90000¹	-	0.22
Cy3B NHS ester	560	571	120000¹	0.048	0.069
XFD405 NHS Ester [equivalent to Alexa Fluor™ 405 NHS Ester]	401	421	35,000	0.23	0.70
CypHer5E NHS Ester	643	660	-	-	-
CypHer7E NHS Ester	748	769	-	-	-
XFD660 NHS Ester [equivalent to Alexa Fluor® 660 NHS Ester]	663	691	132000	0.00	0.10
XFD430 NHS ester	432	540	15,000	-	0.28
XFD610 NHS Ester	611	629	144,000	0.43	0.44
XFD633 NHS Ester	632	650	159,000	-	0.55
XFD660 NHS Ester	663	691	132000	0.00	0.10
Show More (17)

Citations

View all 17 citations: Citation Explorer

High-sensitivity small-molecule detection of microcystin-LR cyano-toxin using a terahertz-aptamer biosensor
Authors: Mohamed, Ahmed and Walsh, Ryan and Cherif, Mohamed and Hafez, Hassan A and Ropagnol, Xavier and Vidal, Fran{\c{c}}ois and Perreault, Jonathan and Ozaki, Tsuneyuki
Journal: Analyst (2021): 7583--7592

The one-pot nonhydrolysis Staudinger reaction and Staudinger or SPAAC ligation
Authors: Cheng, L., Kang, X., Wang, D., Gao, Y., Yi, L., Xi, Z.
Journal: Org Biomol Chem (2019): 5675-5679

Optimization of Chemoenzymatic Mass Tagging by Strain-Promoted Cycloaddition (SPAAC) for the Determination of O-GlcNAc Stoichiometry by Western Blotting
Authors: Darabedian, N., Thompson, J. W., Chuh, K. N., Hsieh-Wilson, L. C., Pratt, M. R.
Journal: Biochemistry (2018): 5769-5774

Highly reactive bis-cyclooctyne-modified diarylethene for SPAAC-mediated cross-linking
Authors: Strizhak, A. V., Sharma, K., Babii, O., Afonin, S., Ulrich, A. S., Komarov, I. V., Spring, D. R.
Journal: Org Biomol Chem (2018): 8559-8564

Fused Bicyclic Caspase-1 Inhibitors Assembled by Copper-Free Strain-Promoted Alkyne-Azide Cycloaddition (SPAAC)
Authors: Qian, L., Zhang, C. J., Wu, J., Yao, S. Q.
Journal: Chemistry (2017): 360-369

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