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Digoxigenin NHS ester
Digoxigenin (DIG) is a hapten, a small molecule with high antigenicity, which is used in many biological detections similarly to other popular haptens such as 2,4-Dinitrophenol (DNP) and biotin. Typically, digoxigenin is conjugated to a macrobiomolecule (e.g., proteins, nucleic acids, etc) to be detected. Anti-digoxigenin antibodies are labeled with fluorescent dyes or enzymes, directly or secondarily, for visualization and detection. Digoxigenin NHS ester is an activated ester which readily reacts with amino groups under mild conditions, attaching the digoxigenin moiety to proteins or amino-substituted oligonucleotides. Digoxigenin-labled biomolecules are used for biological detections in combination with anti-digoxigenin antibodies of high affinity and specificity.
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Cy2DIGE NHS ester | 492 | 508 | 150000 | 0.120 | 0.08 | 0.15 |
| Cy3B NHS ester | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
| Cy3DIGE NHS ester | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
| Cy5DIGE NHS ester | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
| CypHer5E NHS Ester | 643 | 660 | - | - | - | - |
| QXY21 NHS ester [equivalent to QSY-21 NHS ester] | - | - | 890001 | - | - | 0.32 |
| QXY7 NHS ester [equivalent to QSY-7 NHS ester] | - | - | 900001 | - | - | 0.22 |
| XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester* | 343 | 441 | 19000 | - | 0.25 | 0.19 |
| XFD430 NHS ester | 432 | 540 | 15,000 | - | - | 0.28 |
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Citations
View all 21 citations: Citation Explorer
LncRNA AL592284. 1 facilitates proliferation and metastasis of cervical cancer cells via miR-30a-5p/Vimentin/EMT axis
Authors: Zhang, Jing and Liu, Hong-li and Liu, Jing-bo and Zhang, Yuan and Liu, Jing and Li, Yan-hua
Journal: Biochemical and Biophysical Research Communications (2021): 95--102
Authors: Zhang, Jing and Liu, Hong-li and Liu, Jing-bo and Zhang, Yuan and Liu, Jing and Li, Yan-hua
Journal: Biochemical and Biophysical Research Communications (2021): 95--102
A smartphone readout system for gold nanoparticle-based lateral flow assays: application to monitoring of digoxigenin
Authors: Ruppert, C., Phogat, N., Laufer, S., Kohl, M., Deigner, H. P.
Journal: Mikrochim Acta (2019): 119
Authors: Ruppert, C., Phogat, N., Laufer, S., Kohl, M., Deigner, H. P.
Journal: Mikrochim Acta (2019): 119
Application of Antibody-Powered Triplex-DNA Nanomachine to Electrochemiluminescence Biosensor for the Detection of Anti-Digoxigenin with Improved Sensitivity Versus Cycling Strand Displacement Reaction
Authors: Yang, S. S., Jiang, M. H., Chai, Y. Q., Yuan, R., Zhuo, Y.
Journal: ACS Appl Mater Interfaces (2018): 38648-38655
Authors: Yang, S. S., Jiang, M. H., Chai, Y. Q., Yuan, R., Zhuo, Y.
Journal: ACS Appl Mater Interfaces (2018): 38648-38655
Synthesis of C3-Neoglycosides of digoxigenin and their anticancer activities
Authors: Li, X. S., Ren, Y. C., Bao, Y. Z., Liu, J., Zhang, X. K., Zhang, Y. W., Sun, X. L., Yao, X. S., Tang, J. S.
Journal: Eur J Med Chem (2018): 252-262
Authors: Li, X. S., Ren, Y. C., Bao, Y. Z., Liu, J., Zhang, X. K., Zhang, Y. W., Sun, X. L., Yao, X. S., Tang, J. S.
Journal: Eur J Med Chem (2018): 252-262
The cardenolides strophanthidin, digoxigenin and dihydroouabain act as activators of the human RORgamma/RORgammaT receptors
Authors: Karas, K., Salkowska, A., Walczak-Drzewiecka, A., Ryba, K., Dastych, J., Bachorz, R. A., Ratajewski, M.
Journal: Toxicol Lett (2018): 314-324
Authors: Karas, K., Salkowska, A., Walczak-Drzewiecka, A., Ryba, K., Dastych, J., Bachorz, R. A., Ratajewski, M.
Journal: Toxicol Lett (2018): 314-324
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