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2’,3’-cGAMP-iFluor 647 conjugate
iFluor® 647-labeled cGAMP provides an excellent fluorescent probe for developing fluorescence-based assays and tests for detecting cGAMP or monitoring its activity. iFluor® 647 conjugates are well excited with a red laser and can be monitored with the common Cy5 filter set. Additionally, the fluorescence of iFluor® 647 is not affected by pH (4-10). 2’,3’-cGAMP has gained significant attention in recent years due to its potential as a therapeutic target for diseases such as cancer and viral infections. It has been shown to activate the immune system and enhance the efficacy of immune checkpoint inhibitors, which are a type of cancer immunotherapy. 2’,3’-cGAMP (cyclic GMP-AMP) is a cyclic dinucleotide second messenger molecule that plays a critical role in the innate immune system. It is synthesized by the enzyme cGAS (cyclic GMP-AMP synthase) in response to cytosolic DNA that has been released from damaged or infected cells. Once synthesized, 2’,3’-cGAMP binds to the adaptor protein STING (stimulator of interferon genes) and triggers downstream signaling pathways that result in the production of type I interferons and other cytokines, leading to an immune response against the invading pathogen.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 2’,3’-cGAMP-iFluor 647 conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 51.54 µL | 257.7 µL | 515.4 µL | 2.577 mL | 5.154 mL |
| 5 mM | 10.308 µL | 51.54 µL | 103.08 µL | 515.4 µL | 1.031 mL |
| 10 mM | 5.154 µL | 25.77 µL | 51.54 µL | 257.7 µL | 515.4 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| 2’,3’-cGAMP-iFluor 488 conjugate | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
References
View all 16 references: Citation Explorer
Naringenin is a Potential Immunomodulator for Inhibiting Liver Fibrosis by Inhibiting the cGAS-STING Pathway.
Authors: Chen, Li and Xia, Siwei and Wang, Shuqi and Zhou, Yuanyuan and Wang, Feixia and Li, Zhanghao and Li, Yang and Kong, Desong and Zhang, Zili and Shao, Jiangjuan and Xu, Xuefen and Zhang, Feng and Zheng, Shizhong
Journal: Journal of clinical and translational hepatology (2023): 26-37
Authors: Chen, Li and Xia, Siwei and Wang, Shuqi and Zhou, Yuanyuan and Wang, Feixia and Li, Zhanghao and Li, Yang and Kong, Desong and Zhang, Zili and Shao, Jiangjuan and Xu, Xuefen and Zhang, Feng and Zheng, Shizhong
Journal: Journal of clinical and translational hepatology (2023): 26-37
Increased LCN2 (lipocalin 2) in the RPE decreases autophagy and activates inflammasome-ferroptosis processes in a mouse model of dry AMD.
Authors: Gupta, Urvi and Ghosh, Sayan and Wallace, Callen T and Shang, Peng and Xin, Ying and Nair, Archana Padmanabhan and Yazdankhah, Meysam and Strizhakova, Anastasia and Ross, Mark A and Liu, Haitao and Hose, Stacey and Stepicheva, Nadezda A and Chowdhury, Olivia and Nemani, Mihir and Maddipatla, Vishnu and Grebe, Rhonda and Das, Manjula and Lathrop, Kira L and Sahel, José-Alain and Zigler, J Samuel and Qian, Jiang and Ghosh, Arkasubhra and Sergeev, Yuri and Handa, James T and St Croix, Claudette M and Sinha, Debasish
Journal: Autophagy (2023): 92-111
Authors: Gupta, Urvi and Ghosh, Sayan and Wallace, Callen T and Shang, Peng and Xin, Ying and Nair, Archana Padmanabhan and Yazdankhah, Meysam and Strizhakova, Anastasia and Ross, Mark A and Liu, Haitao and Hose, Stacey and Stepicheva, Nadezda A and Chowdhury, Olivia and Nemani, Mihir and Maddipatla, Vishnu and Grebe, Rhonda and Das, Manjula and Lathrop, Kira L and Sahel, José-Alain and Zigler, J Samuel and Qian, Jiang and Ghosh, Arkasubhra and Sergeev, Yuri and Handa, James T and St Croix, Claudette M and Sinha, Debasish
Journal: Autophagy (2023): 92-111
Mechanisms of phase-separation-mediated cGAS activation revealed by dcFCCS.
Authors: Yao, Yirong and Wang, Wenjuan and Chen, Chunlai
Journal: PNAS nexus (2022): pgac109
Authors: Yao, Yirong and Wang, Wenjuan and Chen, Chunlai
Journal: PNAS nexus (2022): pgac109
Therapeutic targeting of STING-TBK1-IRF3 signalling ameliorates chronic stress induced depression-like behaviours by modulating neuroinflammation and microglia phagocytosis.
Authors: Duan, Na and Zhang, Yanpeng and Tan, Shuwen and Sun, Jianyu and Ye, Mao and Gao, Hui and Pu, Kairui and Wu, Meiyan and Wang, Qiang and Zhai, Qian
Journal: Neurobiology of disease (2022): 105739
Authors: Duan, Na and Zhang, Yanpeng and Tan, Shuwen and Sun, Jianyu and Ye, Mao and Gao, Hui and Pu, Kairui and Wu, Meiyan and Wang, Qiang and Zhai, Qian
Journal: Neurobiology of disease (2022): 105739
Chemical Synthesis of the Fluorescent, Cyclic Dinucleotides cth GAMP.
Authors: Veth, Simon and Fuchs, Adrian and Özdemir, Dilara and Dialer, Clemens and Drexler, David Jan and Knechtel, Fabian and Witte, Gregor and Hopfner, Karl-Peter and Carell, Thomas and Ploetz, Evelyn
Journal: Chembiochem : a European journal of chemical biology (2022): e202200005
Authors: Veth, Simon and Fuchs, Adrian and Özdemir, Dilara and Dialer, Clemens and Drexler, David Jan and Knechtel, Fabian and Witte, Gregor and Hopfner, Karl-Peter and Carell, Thomas and Ploetz, Evelyn
Journal: Chembiochem : a European journal of chemical biology (2022): e202200005
Page updated on November 20, 2024
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