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2',3'-cGAMP-Cy5 conjugate
2',3'-cGAMP or cGAMP (Cyclic guanosine monophosphate"adenosine monophosphate) is produced in mammalian cells by cGAS (cGAMP synthase) from ATP and GTP upon cytosolic DNA stimulation. cGAMP produced by cGAS contains mixed phosphodiester linkages, with one between 2\'-OH of GMP and 5\'-phosphate of AMP and the other between 3\'-OH of AMP and 5\'-phosphate of GMP. 2',3'-cGAMP is also referred to as "noncanonical" cGAMP due to the presence of the atypical 2'-5' phosphodiester linkage between the guanosine and the adenosine. Similar to the canonical 3',3'-cGAMP, 2'3'-cGAMP serves as a second messenger to activate innate immune responses by binding to STING (stimulator of IFN genes) and subsequently inducing the TBK1-IRF3-dependent production of IFN-β. This Cy5-labeled cGAMP provides an excellent probe for developing fluorescence-based assays and test for detecting cGAMP and monitoring its activity.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 2',3'-cGAMP-Cy5 conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 51.54 µL | 257.7 µL | 515.4 µL | 2.577 mL | 5.154 mL |
| 5 mM | 10.308 µL | 51.54 µL | 103.08 µL | 515.4 µL | 1.031 mL |
| 10 mM | 5.154 µL | 25.77 µL | 51.54 µL | 257.7 µL | 515.4 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Citations
View all 56 citations: Citation Explorer
Delivery of a STING Agonist Using Lipid Nanoparticles Inhibits Pancreatic Cancer Growth
Authors: Shaji, Sherin George and Patel, Pratikkumar and Mamani, Umar-Farouk and Guo, Yuhan and Koirala, Sushil and Lin, Chien-Yu and Alahmari, Mohammed and Omoscharka, Evanthia and Cheng, Kun
Journal: International Journal of Nanomedicine (2024): 8769--8778
Authors: Shaji, Sherin George and Patel, Pratikkumar and Mamani, Umar-Farouk and Guo, Yuhan and Koirala, Sushil and Lin, Chien-Yu and Alahmari, Mohammed and Omoscharka, Evanthia and Cheng, Kun
Journal: International Journal of Nanomedicine (2024): 8769--8778
African Swine Fever Virus EP364R and C129R Target Cyclic GMP-AMP To Inhibit the cGAS-STING Signaling Pathway
Authors: Dodantenna, Niranjan and Ranathunga, Lakmal and Chathuranga, WA Gayan and Weerawardhana, Asela and Cha, Ji-Won and Subasinghe, Ashan and Gamage, Nuwan and Haluwana, DK and Kim, YongKwan and Jheong, WeonHwa and others,
Journal: Journal of Virology (2022): e01022--22
Authors: Dodantenna, Niranjan and Ranathunga, Lakmal and Chathuranga, WA Gayan and Weerawardhana, Asela and Cha, Ji-Won and Subasinghe, Ashan and Gamage, Nuwan and Haluwana, DK and Kim, YongKwan and Jheong, WeonHwa and others,
Journal: Journal of Virology (2022): e01022--22
A Click-Chemistry Linked 2'3'-cGAMP Analogue
Authors: Dialer, C. R., Stazzoni, S., Drexler, D. J., Muller, F. M., Veth, S., Pichler, A., Okamura, H., Witte, G., Hopfner, K. P., Carell, T.
Journal: Chemistry (2019): 2089-2095
Authors: Dialer, C. R., Stazzoni, S., Drexler, D. J., Muller, F. M., Veth, S., Pichler, A., Okamura, H., Witte, G., Hopfner, K. P., Carell, T.
Journal: Chemistry (2019): 2089-2095
Viral and metazoan poxins are cGAMP-specific nucleases that restrict cGAS-STING signalling
Authors: Eaglesham, J. B., Pan, Y., Kupper, T. S., Kranzusch, P. J.
Journal: Nature (2019): 259-263
Authors: Eaglesham, J. B., Pan, Y., Kupper, T. S., Kranzusch, P. J.
Journal: Nature (2019): 259-263
Structure and mechanism of a Hypr GGDEF enzyme that activates cGAMP signaling to control extracellular metal respiration
Authors: Hallberg, Z. F., Chan, C. H., Wright, T. A., Kranzusch, P. J., Doxzen, K. W., Park, J. J., Bond, D. R., Hammond, M. C.
Journal: Elife (2019): se name="20310.enl" path="C:\Users\aatbi\Drop
Authors: Hallberg, Z. F., Chan, C. H., Wright, T. A., Kranzusch, P. J., Doxzen, K. W., Park, J. J., Bond, D. R., Hammond, M. C.
Journal: Elife (2019): se name="20310.enl" path="C:\Users\aatbi\Drop
Page updated on December 17, 2024
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