10XHis maleimide
10XHis maleimide is an excellent building block to make 10XHis conjugates for developing His tag detection probes and purification tools. It readily reacts with a biomolecule that contains a thiol group such as antibodies, peptides and thiol-modified oligos. The 10XHis tag is one of the most common His tags and has a molecular weight of ~2kDa. His-tags (i.e., polyhistidine tags) comprise a consecutive series of six to ten histidine residues. His tags are used for many recombinant proteins to facilitate purification, allowing researchers to extract a protein of interest from thousands of other proteins found in a cell or cell lysate. The small size of the 10X-His tag has a lower possibility for the tag to affect the functionality of the fusion protein. Histidine forms coordination bonds with immobilized transition metal ions, and this property can be utilized for protein purification. His-tag protein purification is by a specialized form of affinity chromatography, called immobilized metal affinity chromatography (IMAC), where proteins or peptides are separated according to their affinity for metal ions immobilized to a solid chelating resin. During this process, a small His-tag is fused to either the N or C terminus of the target protein, enabling capture by nickel or cobalt ions coordinated on a variety of resins. The small size, low cost, and ease of use have made the His-tag the most popular affinity-tag available. AAT Bioquest offers the largest collection of 6XHis, 0XHis, NTA, bis-NTA, tris-NTA and IDA reagents for His Tag detections and purifications.
References
View all 44 references: Citation Explorer
Selective APC-targeting of a novel Fc-fusion multi-immunodominant recombinant protein (tTax-tEnv:mFcγ2a) for HTLV-1 vaccine development.
Authors: Shafifar, Mina and Mozhgani, Sayed-Hamidreza and Razavi Pashabayg, Kobra and Mosavat, Arman and Karbalaei, Mohsen and Norouzi, Mehdi and Rezaee, Seyed Abdolrahim
Journal: Life sciences (2022): 120920
Authors: Shafifar, Mina and Mozhgani, Sayed-Hamidreza and Razavi Pashabayg, Kobra and Mosavat, Arman and Karbalaei, Mohsen and Norouzi, Mehdi and Rezaee, Seyed Abdolrahim
Journal: Life sciences (2022): 120920
Reshaping nanobodies for affinity purification on protein a.
Authors: Crauwels, Maxine and Van Vaerenbergh, Nele and Kulaya, Neeme Benedict and Vincke, Cécile and D'Huyvetter, Matthias and Devoogdt, Nick and Muyldermans, Serge and Xavier, Catarina
Journal: New biotechnology (2020): 20-28
Authors: Crauwels, Maxine and Van Vaerenbergh, Nele and Kulaya, Neeme Benedict and Vincke, Cécile and D'Huyvetter, Matthias and Devoogdt, Nick and Muyldermans, Serge and Xavier, Catarina
Journal: New biotechnology (2020): 20-28
Simplified detection of polyhistidine-tagged proteins in gels and membranes using a UV-excitable dye and a multiple chelator head pair.
Authors: Raducanu, Vlad-Stefan and Isaioglou, Ioannis and Raducanu, Daniela-Violeta and Merzaban, Jasmeen S and Hamdan, Samir M
Journal: The Journal of biological chemistry (2020): 12214-12223
Authors: Raducanu, Vlad-Stefan and Isaioglou, Ioannis and Raducanu, Daniela-Violeta and Merzaban, Jasmeen S and Hamdan, Samir M
Journal: The Journal of biological chemistry (2020): 12214-12223
A bispecific circular aptamer tethering a built-in universal molecular tag for functional protein delivery.
Authors: Pan, Xiaoshu and Yang, Yu and Li, Long and Li, Xiaowei and Li, Qiang and Cui, Cheng and Wang, Bang and Kuai, Hailan and Jiang, Jianhui and Tan, Weihong
Journal: Chemical science (2020): 9648-9654
Authors: Pan, Xiaoshu and Yang, Yu and Li, Long and Li, Xiaowei and Li, Qiang and Cui, Cheng and Wang, Bang and Kuai, Hailan and Jiang, Jianhui and Tan, Weihong
Journal: Chemical science (2020): 9648-9654
The surface syndecan protein from Macrobrachium rosenbergii could function as mediator in bacterial infections.
Authors: Yang, Hui and Xiong, Haoran and Mi, Kaihang and Zhang, Yingying and Zhang, Xiaojun and Chen, Guohong
Journal: Fish & shellfish immunology (2020): 62-68
Authors: Yang, Hui and Xiong, Haoran and Mi, Kaihang and Zhang, Yingying and Zhang, Xiaojun and Chen, Guohong
Journal: Fish & shellfish immunology (2020): 62-68
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