Calculator
DNA Concentration Calculator
The concentration of DNA in solution can be determined by substituting the molecular weight, extinction coefficient and λ max into a derived form of the Beer - Lambert Law. A substance's λmax is the wavelength at which it experiences the strongest absorbance. For DNA, this wavelength is 260 nm.
The absorbance at λmax can be measured using a spectrophotometer. There are some important things to keep in mind when measuring 260 absorbance:
The absorbance at λmax can be measured using a spectrophotometer. There are some important things to keep in mind when measuring 260 absorbance:
- The DNA should be well-dissolved in solution. DNA precipitation will cause inaccuracies in concentration calculations.
- The absorbance reading should not exceed the maximum detection limit of the instrumentation. This can be identified by a plateau in the absorbance spectrum. If the absorbance spectrum plateaus, dilute the sample and try again.
- This calculator assumes a 1 cm pathlength for instrumentation. Standard cuvettes for spectrophotometers are typically 1 cm. If a different pathlength is used, value should be corrected during data entry.
How to use this tool
- Select the DNA type. Or select "custom sequence" to enter the nucleic acid sequence.
- Enter the absorbance at λmax. For a typical nucleic acid sequence, λmax is 260 nm. However, this value may change based on the sequence.Please use the maximum absorbance as indicated by the spectrophotometric reading (ie. highest peak).
- If the absorbance is obtained with a diluted sample, enter the dilution factor in order to determine the concentration of the original sample.
- If pathlength differs from 1 cm, enter corrected value into pathlength textbox.
- Press calculate to display the concentration.
Data Entry
| DNA | |
| Absorbance at λmax | |
| Dilution factor (optional) | |
| Pathlength | cm |
Feedback
Have a question or a feature request about this tool? Feel free to reach out to us and let us know! We're always looking for ways to improve!References
This online tool may be cited as follows
MLA | "Quest Calculate™ DNA Concentration Calculator." AAT Bioquest, Inc., 22 Nov. 2024, https://www.aatbio.com/tools/calculate-DNA-concentration. | |
APA | AAT Bioquest, Inc. (2024, November 22). Quest Calculate™ DNA Concentration Calculator. AAT Bioquest. https://www.aatbio.com/tools/calculate-DNA-concentration. | |
| BibTeX | EndNote | RefMan |