Why is it important in next generation sequencing (NGS)?

DNA normalization is important in NGS for several reasons: 

• Normalization helps maximize the use of NGS technology by allowing researchers to run multiple libraries on a single flow cell, driving down costs. 
• Inconsistent concentrations of DNA fragments obtained from different sample types and qualities can lead to confusing research results due to discrepancies in data quality, resulting in repeated experiments, additional work time, and higher costs. DNA normalization ensures that researchers obtain reliable, consistent data, which is vital for clinical NGS.
• Normalization reduces noise and generates higher average reads per sample with even more sequencing depth, thus improving the efficiency of NGS. 
• DNA normalization enhances the accuracy and reliability of downstream analyses such as PCR, protein assays, or sequencing. This reduces the likelihood of clinicians missing key information such as a single nucleotide variation or a rare allele, which could lead to a misdiagnosis.