Why are dilutions typically made in a series rather than a single large dilution?

Making serial dilutions is simpler and ensures a gradual and consistent reduction in concentration across a wide range compared to doing a single large dilution. In a series of dilutions, each step has the same fold-dilution factor, creating a consistent geometric process with a constant ratio between every pair of consecutive dilutions. Additionally, serial dilutions permit more precise regulation over the dilution factor. This is due to the stepwise reduction in concentration, allowing for a more accurate estimation of the initial sample’s concentration. Subsequently, errors in steps during serial dilution can be fixed without affecting the entire dilution process. In contrast, if a mistake occurs in a single large dilution, it may significantly affect the final result.