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AAT Bioquest

Which DNA polymerase is most fidelity-oriented?

Posted August 15, 2024


Answer

DNA polymerase III is considered the most fidelity-oriented of the three polymerases because it facilitates precise DNA replication with fewer mistakes compared to DNA polymerase I and DNA polymerase II. This is because DNA polymerase III possesses a 3' to 5' exonuclease activity, which allows it to remove incorrect nucleotides immediately after they are added. 

Additional resources

Fidelity of DNA replication—a matter of proofreading

DNA and RNA Quantitation

Helixyte™ Green Fluorimetric Total Nucleic Acid Quantitation Kit *Optimized for Microplate Readers*