When should I use western blotting for protein analysis?

Western blotting is a laboratory technique used to detect and quantify specific proteins from a complex mixture or sample. It is highly specific in measuring even relatively low levels of proteins.

Western blotting is the best method for protein analysis under these circumstances: 

• You need to detect a variety of proteins, particularly in complex samples such as cell lysates: Fluorescent multiplexing can enhance your analysis by allowing the detection of multiple targets simultaneously, with some advanced tools allowing you to capture up to three target proteins simultaneously using three-color fluorescence.
• You want to identify specific proteins: Western blots can accurately detect a target protein in a sample, even at low abundance or within complex mixtures.
• You need to confirm you’re measuring the correct protein: Western blotting verifies proteins based on size and other factors and can also expose modifications, impurities, and more.