When should I use ELISA for protein analysis?

ELISA or enzyme-linked immunosorbent assay is a highly sensitive and specific enzyme immunoassay technique for qualitative and quantitative analysis of antibodies and antigens including but not limited to nucleic acids, proteins, peptides, and hormones. 

ELISA is the best method for protein analysis under these circumstances: 

• Detecting rare or low-abundance proteins or trace amounts of target proteins: Its exceptional sensitivity makes ELISA ideal for this purpose. 
• Precise quantification of target protein in a sample is required: ELISA is an effective technique for measuring protein concentrations and tracking variations in protein levels over time.
• You're working under time constraints in the lab: ELISA assays are fast, straightforward, and require minimal sample preparation, making them an ideal choice for routine testing and quick analysis of samples. ​