When should I use dPCR to quantify NGS libraries?
Posted August 14, 2023
Answer
Digital PCR (dPCR) is particularly useful for quantifying non-standard library protocols, which may have a higher population of incorrectly formed constructs. In non-standard libraries, dPCR offers a simple, accurate and effective way to quantify NGS (Next-Generation Sequencing) libraries, while avoiding potential errors that can occur with other techniques such as spectrophotometry or microfluidic electrophoresis.
Additional resources
DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1*
Tide Fluor™ 1 acid [TF1 acid] *Superior replacement for EDANS*