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AAT Bioquest

When is buffer capacity at maximum?

Posted December 22, 2023


Answer

Buffer capacity is at maximum when the concentration of the weak acid and its salt or weak base and its salt are equal. In other words, Salt=Acid (in acid buffer), and Salt=Base (in base buffer). In equation terms it looks like the following: pH=PKa or pOH=pKb..  

Additional resources

Buffer Capacity and Buffer Range

Buffers and Lab Consumables

ReadiUse™ mammalian cell lysis buffer *5X*