What should I take into consideration when diluting ethanol?

There are several things one should consider when diluting ethanol. One important factor to consider is the desired concentration of the ethanol. In a 70% ethanol solution, the longer evaporation time compared to higher concentrations allows for more effective penetration into cells. Additionally, 70% tends to be more cost-effective compared to higher concentrations and ethanol is less flammable. 

The water content in the 70% solution plays a crucial role in denaturing proteins. The prolonged contact time enhances its effectiveness as it can penetrate cells more efficiently. One should also consider the proper diluent or solvent they are using. Distilled water is commonly used to dilute ethanol. Another factor one should consider is to use precise measuring instruments such as volumetric flasks or pipettes to ensure accurate dilution. Additionally, fluctuations in temperature and environmental conditions can influence the accuracy of dilutions. Thus, it is important to carry dilutions in a controlled temperature environment and allow solutions to reach the intended temperature before measurements are taken. Lastly, ethanol is highly flammable, and thus dilution procedures should be done in a well-ventilated area like a fume hood.