What should be considered when I do in-gel staining?

When doing in-gel staining, it’s advisable to compare the benefits and limitations of the technique. 

Benefits: In gel staining is more convenient, has a faster workflow, and requires less stain for visualization. 

Limitations: Sample mobility may be altered and the samples may not run true to size because of the dye binding to nucleic acids. This can make it difficult to detect samples of lower molecular weight, especially during a long run. Moreover, dyes can only be used once and may run off after a long run.